摘要
背景:硫化氢具有舒张离体血管平滑肌、促进下丘脑促肾上腺皮质激素释放激素和细胞膜超极化的作用。中枢内硫化氢是否参与心血管活动的调节目前尚未得到充分的研究。目的:观察中枢内源性硫化氢的心血管效应及其机制。设计、时间及地点:随机对照动物实验,于2007-10/2008-10在解放军第一医院检验科完成。材料:清洁级雄性SD大鼠40只,体质量200～250g;S-腺苷蛋氨酸,KATP通道阻断剂格列苯脲为Sigma公司产品。方法:40只大鼠随机被分为6组:①对照组6只,注射人工脑脊液。②S-腺苷蛋氨酸低剂量组6只注射S-腺苷蛋氨酸5μmol、中剂量组5只注射S-腺苷蛋氨酸10μmol、高剂量组7只注射S-腺苷蛋氨酸20μmol。③预先注射人工脑脊液8只或格列苯脲格列苯脲8只,40min后注射10μmolS-腺苷蛋氨酸。将大鼠固定于立体定位仪,进行侧脑室注射。主要观察指标:①侧脑室注射不同剂量S-腺苷蛋氨酸后对血压及心率的影响。②侧脑室注射KATP通道阻断剂格列苯脲对侧脑室注射S-腺苷蛋氨酸中枢心血管效应的影响。结果:侧脑室注射S-腺苷蛋氨酸能够显著降低麻醉大鼠的血压,减慢大鼠的心率。侧脑室注射人工脑脊液不影响麻醉大鼠的血压和心率。格列苯脲预处理能显著减弱侧脑室10μmolS-腺苷蛋氨酸产生的降低血压效应,与人工脑脊液预处理组比较差异有显著性意义(P<0.05),格列苯脲+S-腺苷蛋氨酸组和对照组比较差异无显著性意义(P>0.05)。结论:侧脑室注射S-腺苷蛋氨酸增加内源性硫化氢产生的中枢性降低血压、减慢心率的作用可能与KATP通道开放有关。
BACKGROUND： Hydrogen sulphide has been considered as a novel member of gasotransmitter family with important roles in relaxation of vascular smooth muscle, release of the corticotrophin-releasing hormone and hyperpolarization of cell membranes. However, the central cardiovascular functions of hydrogen sulphide are not fully identified. OBJECTIVE： To explore the cardiovascular effects and mechanism of endogenous hydrogen sulphide in central nervous systems. DESIGN, TIME AND SETTING： Randomized control animal experiment was performed in the Department of Clinical Laboratory, First Hospital of Chinese PLA between October 2007 and October 2008. MATERIALS： Forty male SD rats of clean grade, weighing 220-250 g; S-ademetionine （SAM）, KATP channel blocker glibenclamide was purchased from Sigma. METHODS： Forty male anesthetized SD rats were divided into six groups at random： ①Control group （n=6） was injected with artificial cerebrospinal fluid （aCSF）. ②Low-dose SAM group （n=6） was injected with 5 μmol SAM, medium-dose SAM group （n=5） with 10 μmol SAM and high-dose SAM group （n=7） with 20 μmol SAM. ③aCSF （n=8） or glibenclamide （n=8） were correspondingly prior intracerebroventricular injection applied in 16 rats, 10 μmol SAM was followed 40 minutes later. All rats were fixed on stereotaxic apparatus to receive the lateral cerebral ventricle injection. MAIN OUTCOME MEASURES： ①The effects of intracerebroventricular injection of different doses of SAM on the blood pressure and heart rate. ②The influences of prior application of into lateral cerebral ventricle injection of KATP channel block glibenclamide on the cardiovascular effect of SAM. RESULTS： Intracerebroventricular injection of SAM produced a significant decrease in blood pressure and heart rate of the anesthetized rats. The aCSF injection had no influence on these two indices. The cardiovascular effects of lateral cerebral ventricle injection of SAM （10 μmol） were completely attenuated by KATP blocker
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第33期6527-6530,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30700266)
陕西省卫生厅资助项目(06E05)~~
