摘要
目的:比较硫酸乙酰肝素蛋白聚糖(简称HSPG)和去硫酸乙酰肝素链(简称HS-GAGs链即HS链)的HSPG的体外抗人乳腺癌细胞MDA-MB-231的抗增殖和凋亡诱导作用。方法:对HSPG进行提取、纯化,硫酸乙酰肝素酶1水解HSPG的HS链;光学显微镜法观察两组分引起的细胞形态学的变化;MTT比色法检测两组分对肿瘤细胞生长曲线的影响;流式细胞术结合Annexin V-FITC及PI双染标记法观察两组分对肿瘤细胞凋亡的影响。结果:HSPG可致MDA-MB-231细胞脱落悬浮;MTT法检测结果显示HSPG对MDA-MB-231细胞增殖的抑制作用随浓度的增加而增强,而去HS链HSPG只有在高浓度时才呈现出抑制作用。HSPG能诱导MDA-MB-231的早期凋亡,而去HS链HSPG在高浓度(4 mg/mL)时对MDA-MB-231早期凋亡有诱导作用。结论:HSPG能诱导MDA-MB-231的早期凋亡和抑制细胞生长增殖。作用机制可能与HSPG的硫酸乙酰肝素链有关。
AIM： To compare the antiproliferative effect and apoptosis-inducing activity by HSPG and HSPG without heparan sulfate chains on htunan mammary cancer cell MDA-MB-231 in vitro. METHODS： HSPG was isolated and purified, the heparan sulfate chains of HSPG was hydrolyzed by heparitinasel. The changes of two components cell morphology were observed by light microscope, the anti-proliferation of HSPG and HSPG without HS chains was measured by MTT assay, the cell growth curve was drawn and the apoptosis was detected by Annexin V/PI double staining and flow cytometry. RESULTS： MCF-7 type HSPG induced the exfoliation and suspension of MDA-MB- 231 cells. MTT assay showed that different concentrations of HSPG inhibited MDA-MB-231 cells prolifemtion in a concentration-dependent manner. There was inhibition effect only in high concentrations of HSPG without HS chains. HSPG induced MDA-MB-231 cells＇ apoptosis at the early stage,however, 4 mg/mL HSPG without HS chains had some effects on the apoptosis at the early stage. CONCLUSION： HSPG could induce MDA-MB-231 cells＇ apoptosis at the early stage and distinctly inhibit the growth of MDA-MB-231 cells, the mechanism may be related to HS chains of HSPG.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第6期617-623,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
