摘要
应用免疫组化（ＳＰ法）和革兰氏染色技术，对３６例胆囊腺癌和３０例慢性胆囊炎进行ｐ２１、ｐ５３蛋白及细菌Ｌ型检测，同时对Ｌ型阳性和阴性组织的ｐ２１、ｐ５３表达进行对比分析。结果发现，腺癌和慢性炎的革兰染色Ｌ型检出率分别为８０．６％和７６．７％，与Ｌ型抗原检出率均无显著性差异（Ｐ＞０．０５）。腺癌组的ｐ２１、ｐ５３表达率明显高于炎症组（Ｐ＜０．００５），腺癌中Ｌ型阳性组的ｐ２１、ｐ５３表达率也高于其Ｌ型阴性组（Ｐ＜０．０５）。表明Ｌ型感染与胆囊腺癌、慢性炎及ｐ２１、ｐ５３过表达有关，ｐ２１、ｐ５３过表达与胆囊腺癌也有关。提示Ｌ型参与了ｒａｓ、ｐ５３基因突变，并可能通过致基因突变而成为胆囊腺癌发生的危险因素之一。
36 cases with adenocarcinoma of the gallbladder (ACG) and 30 cases of chronic gallbladditis (CGS) associated with bacterial Lform were examined by Gram’s staining and immunohistochemistry.It showed that the positive rate of the infection of bacterial Lform in ACG group and in CGS group was 806% and 767% respectively.The expression of P21 and P53 were significantly higher in Lform infection positive group than that in negative group(p<0.05).the results indicate that Lform infection was correlated with ACG,CGS and overexpression of P21 and P53.It suggests that ras gene and P53 gene mutation may be related with Lform infection.Bacterial Lform infection may be one of the important factor leading to the ACG.
出处
《临床肝胆病杂志》
CAS
北大核心
1998年第3期161-163,共3页
Journal of Clinical Hepatology
