摘要
采用双抗夹心ＥＬＩＳＡ技术检测了２８例哮喘患儿血浆及外周血单个核细胞（ＰＢＭＣ）在植物血凝素（ＰＨＡ）刺激下产生ＩＬ－１０水平，同时检测了ＮＯ和ＩＬ－２水平。结果：①急性发作期哮喘患儿血浆及ＰＢＭＣ在ＰＨＡ刺激下产生ＩＬ－１０水平均比正常儿童显著降低（Ｐ＜０．０１），在哮喘缓解期虽均有所恢复，但仍低于正常儿童（Ｐ＜０．０５）；②ＮＯ水平在急性发作期患儿明显升高（Ｐ＜０．０５），而在缓解期恢复正常（Ｐ＞０．０５）；③急性发作期哮喘患儿在ＰＨＡ刺激下ＰＢＭＣ产生ＩＬ－２水平明显升高（Ｐ＜０．０１），但在缓解期恢复正常（Ｐ＞０．０５）；④急性发作期哮喘患儿在ＰＨＡ刺激下ＰＢＭＣ产生ＩＬ－１０水平与ＩＬ－２呈显著负相关（ｒ＝－０．５０４，Ｐ＜０．０１），而与血浆ＮＯ水平相关不显著（ｒ＝－０．３１９，Ｐ＞０．０５）。提示：哮喘发作时ＩＬ－１０分泌及释放减少，ＮＯ和ＩＬ－２水平升高，导致或加重气道慢性炎症反应，引起气道高反应性而诱发或加重哮喘。
We used double antibody sandwich ELISA for the quantitative determination of plasma IL10 level and the production of IL2, 10 by PHAactivated peripheral blood mononuclear cells (PBMC) level in 28 asthmatic children and 20 matched normal children. At the same time, the plasma nitrite and nitrate(NO2/NO3) level was determined. Results showed that asthmatic children in acute attack stage(pretherapy) had decreased amounts of the plasma IL10 and the production of IL10 by PHAactivated PBMC compared with normal control subjects (7.21±2.22,11.16±2.88;22.16±5.58,64.11±10.44pg/ml, respectively, both of the P<0.01), while in restoration stage (posttherapy) which had restored in some degree, but still lower than the normal levels(9.25±1.62,56.84±10.43,respectively, P<0.05). Asthmatic children in acute attack stage had increased amounts of the plasma NO2/NO3 and the production of IL2 by PHAactivated PBMC compared with normal control subjects (NO2/NO3:32.67±4.51,29.64±2.93μmol/L;IL2:21357±5978,16152±3411cpm, respectively, P<0.05,0.01 respectively),while in restoration stage which had decreased amounts of the plasma NO2/NO-3 and the production of IL2 by PHAactivated PBMC to normal levels (NO2/NO3:30.18±3.09μmol/L;17068±3514cpm,respectively, both of the P>0.05). Linear correlation analysis showed obvious correlation between the production of IL10 and IL2 by PHAactivated PBMC. The
出处
《免疫学杂志》
CAS
CSCD
北大核心
1998年第3期179-181,共3页
Immunological Journal
基金
第三军医大学大坪医院基金
