摘要
目的利用Livin反义寡核苷酸在裸鼠体内抑制Livin基因表达,探讨Livin反义核苷酸对肝癌裸鼠移植瘤生长的抑制作用。方法设计靶向Livin基因的反义寡核苷酸,以Lipofectamine2000作为载体导入裸鼠皮下移植瘤,观察肿瘤生长情况。采用RT-PCR方法检测实验组和对照组Livin基因表达,TUNEL法检测凋亡指数。结果ASODN组裸鼠皮下移植瘤后14d,肿瘤体积明显变小。LivinmRNA表达明显降低。结论Livin反义寡核苷酸可以明显抑制肝癌裸鼠移植瘤的增殖。
[Objective]To inhibit the livin gene expression in nude mouse by antisense oligonucleotide and to observe the inhibition effect in transplantation tumor of hepatoceUular carcinoma in nude mouse. [Methods] To design and construct the antisense oligonueleotide targeting livin and then transfeeted into the nude mice with transplantation tumor and to observe its growth.To detect the Livin gene expression between the ASODN group, MSODN group and the control group by RT-PCR. [Results] The gross tumor volume became obviously small as well as the expression of livin mRNA lowered after 14 days of the livin ASODN injected into transplantation tumor of nude mice. [Conclusion] The livin ASODN can obviously inhibit the proliferation of transplantation tumor of hepatoceUular carcinoma in nude mice.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第14期2102-2105,共4页
China Journal of Modern Medicine
关键词
LIVIN
寡核苷酸类
反义
细胞凋亡
肝癌
livin
oligonucleotides
antisense
apoptosis
HepatoceUular Carcinoma
