摘要
目的通过对迷迭香酸(RA)作用于结扎大鼠左冠状动脉前降支所致心肌缺血模型的影响,探讨迷迭香酸对心肌缺血的保护作用。方法取120只雄性Wister大鼠随机分为假手术组、缺血模型组、硝酸异山梨酯组、RA小剂量组、RA中剂量组、RA高剂量组。以结扎冠状动脉前降支造成大鼠急性心肌缺血模型,观察心电图变化,检测血清CK-MB、GOT、LDH、cTnT以及细胞内SOD、MDA含量,测定冠脉结扎后心肌梗死面积。结果与假手术组比较,缺血模型组不同时间心电图ST段抬高明显,CK-MB、GOT、LDH、cTnT升高明显,MDA值升高,SOD值减小,心肌梗死明显。所有RA组与缺血模型组比较,不同时间心电图ST段抬高的最大值明显降低,CK-MB、GOT、LDH、cTnT值降低,MDA值减小,SOD值升高,心肌梗死面积明显缩小。结论迷迭香酸对大鼠冠脉结扎后引起的心肌缺血损伤具有保护作用,其机制之一可能与提高氧化酶活性、增强清除自由基能力有关。
Objective Through the influence of Rosmarinic Acid （RA） on the model of myocardial ischemia by descending anterior branch of the left coronary artery ligation in rats, the protective effects of Rosmarinic Acid on myocardial ischemia were investigated in this study. Methods 120 healthy male Wistar rats and divided randomly into six groups： sham group, model group, isosorbide dinitrate group, three Rosmarinic acid （RA） groups with low dosage, middle dosage and high dosage. Acute myocardial ischemia model were made by ligating the descending anterior branch of the left coronary artery. We observed the change of ST -T segment of ECG in different period, the level of CK - MB, GOT, LDH, cTnT in serum, SOD and MDA in cell and the infarction area in ligated heart. Results Compared with the sham group, ST - T segment in ECG in the model group elevated obviously in different period, the level of CK - MB, GOT, LDH, cTnT and MDA increased, value of SOD decreased. The infarction area was found obviously in the ligated heart. In the RA groups, compared with the model group, the maximal values of ST- T segment elevation in ECG decreased clearly, the level of CK- MB, GOT, LDH, cTuT decreased, the value of MDA reduced and the value of SOD increased. The infarction areas in ligated hearts were obviously reduced. Conclusion The RA can prevent rat myocardium from acute ischemic injury. One of the mechanisms may be related to increasing the enzyme activity of anti - xoidation to remove free radicals.
出处
《中国医学创新》
CAS
2009年第22期18-20,共3页
Medical Innovation of China
关键词
迷迭香酸
心肌缺血
血清酶学
超氧化物歧化酶
丙二醛
Rosmarinic Acid
Myocardial ischemia
Enzyme of serum
Superoxide dismutase
Malondialdehyde
