摘要
目的评价不同检测系统红细胞计数(RBC)、血红蛋白(Hb)、红细胞比容(HCT)的偏差及临床可接受范围。方法根据美国临床实验室标准化委员会(NCCLS)EP-9A2文件要求,每天选取8份标本,分别在不同检测系统上进行血常规测定,共测定8d,记录结果。应用SPSS13.0和Excel2000软件包对所测结果进行统计分析,计算和评价各系统间的偏差。以汕头大学第一附属医院Ac.t.5DiffAL为参考检测系统,汕头潮南民生医院Ac.t.5DiffOV为实验检测系统进行方法学比较,并算出两种系统间偏差的相关方程式,分别用RBC、Hb、HCT医学决定水平浓度代入方程,计算相对偏差,以1/3CLIA′88规定的允许误差为标准进行评估。结果两种系统间RBC的相关系数(r)=0.997,回归方程式为^Y=0.987X+0.009;Hb的r=0.999,回归方程式为^Y=0.992X+0.717;HCT的r=0.998,回归方程式为^Y=1.005X-0.002。结论两种系统检测RBC、Hb、HCT的偏差在临床可接受范围内,结果不仅符合临床参考范围,并且具有可比性。
Objective To evaluate the bias and clinical acceptability of RBC,Hbg, Hct results in different detection systems. Methods According to the profile of NCCLS EP-gA2 document,8 samples were chosen to recieve the test of RBC counts, Hbg, Hct in different laboratories in different detection systems in 8 days. Results the results were analysised with data analysis system SPSS 13.0 and Excel2000 ,and evaluate the bias of the two laboratory systems. Ac. t. 5Diff AL in the first Affiliated Hospital of Medical College Shantou University, was set up as the comparison system, Ac. t. 5Diff OV in Chaonan Minsheng hospital was set up as the laboratory system. Relative bias were computed by Medical Decision Level of RBC counts,Hbg, Het,Compare them with 1/3 CLIA＇88 error excepted and evaluate bias of the two laboratories. Results：between the two laboratories systems,the correlation coefficient of RBC is 0. 997,and regression equation is Y= 0. 987X＋0. 009;the correlation coefficient of Hbg is 0. 999, and regression equation is Y= 0. 992X＋0. 717 ;the correlation coefficient of Hct is 0. 998, and regression equation is Y= 1. 005X- 0. 002. Conclusion The bias of RBC counts, Hbg, Hct test between the two laboratory systems were in the clinical acceptable range,and it is of comparability.
出处
《检验医学与临床》
CAS
2009年第16期1328-1329,1331,共3页
Laboratory Medicine and Clinic
