摘要
目的探讨环氧化酶-2(COX-2)抑制剂美洛昔康对角膜新生血管(CNV)的抑制作用及其作用机制。方法缝线法诱导大鼠CNV形成;将动物分5组:第1～3组分别为0.625、1.25、5.0mg/mL美洛昔康组,第4组为地塞米松组,第5组为阴性对照组。裂隙灯照相并计算CNV面积,RT-PCR法观察角膜VEGF和Ang-2 mRNA表达。结果第5组CNV面积明显高于其他各组(P<0.05)。第5天、第8天时,第2组、4组CNV面积明显低于其余各组(P<0.01);第5天时,第4组CNV面积明显低于第2组(P<0.05)。缝线后第8天、第23天,第2～4组Ang-2 mRNA、VEGF mRNA表达均低于第5组。结论COX-2抑制剂美洛昔康可明显抑制CNV的增生,可能通过减少VEGF、Ang-2的转录实现。
Objective Cyclooxygenase-2（COX-2） inhibitor is a new nonsteroidal anti-inflammatory drugs,and its action is to suppress the inflammatory reaction. Present study was to investigate the inhibitory effect of COX-2 inhibitor, meloxicam, on corneal neovascularization（CNV）. Methods Corneal new blood vessel was induced in the right eyes of 50 Wistar rats by scratching in cornea with 3 mm corneal trephine,and then the interrupted suturing was performed at the positions of 3：00,7：00 and 11：00 with 10-0 nylon stitches. 0.05 mL of 0. 625,1.25,5.0 mg/mL meloxican solution was injected subconjunctivally in the model eyes from the end of the operation through 7 days once each day in the lst,2nd,3rd group respectively. The same volume of dexamethasone （2 mg/mL） or solvent was used in the group 4 or group 5. Corneal angiogenesis was clinically evaluated and quantitatively analysed at 5,8,12,16 and 23 days after operation under the slit lamp. Expression of Ang-2 and vascular endothelial growth factor （VEGF） mRNA in the cornea was detected using RT-PCR. Results The new blood bud appeared in the 4th day after operation. The area of new blood vessels was gradually increased and reached peak in the 8th day. The mean area of CNV in group 2,4 was significantly smaller than that of other groups in the 5th and 8th day（P 〈 0.01 ）. On day 5,the mean ares of CNV in group 4 was smaller than that of group 2 （ P 〈 0. 05 ）. On day 8 and 23, the expression of Ang-2 mRNA and VEGF mRNA in group 5 was significantly enhanced in comparison with other groups,and expression of VEGF mRNA followed the same pattern to Ang-2 mRNA. Conclusion Topically administration of meloxicam can inhibit CNV effectively through descending the transcription of Ang-2 and VEGF.
出处
《眼科研究》
CSCD
北大核心
2009年第8期680-683,共4页
Chinese Ophthalmic Research
