摘要
首次采用均匀设计和回归分析方法对影响珙桐(Davidia involucrata)胚萌发的3种因素(基本培养基、赤霉素和活性炭)进行优化,并利用萌发胚建立了珙桐的高效再生体系。利用回归方程得出胚萌发的最优条件为:在含有0.5mg.L-1IBA和1mg.L-16-BA的1/2MS培养基中添加2.85mg.L-1GA3和0.25g.L-1活性炭。在验证实验中珙桐胚萌发率为87.72%,与预测值无显著差异。最优的芽诱导与增殖条件为:添加0.1mg.L-1NAA和2.0mg.L-16-BA的WPM培养基,增殖系数为4.34。在生根培养基中增殖的芽生根率高达90%,移栽成活率为71%。
Three factors （media type, gibberellic acid [GA3] and active carbon） affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis. Then a system for micropropagating D. involucrata was established from the embryos. From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg·L^-1 GA3 and 0.25g·L^-1 activated carbon to 1/2 MS medium containing 0.5 mg·L^-1 IBA and 1 mg·L^-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg·L^-1 NAA and 2.0 mg·L^-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting,
出处
《植物学报》
CAS
CSCD
北大核心
2009年第4期491-496,共6页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.30670209)
四川省教育厅重大培育项目(No.07ZZ015)
四川省科技厅应用基础项目(No.05JY029-107)
关键词
珙桐
胚
回归分析
均匀设计
Davidia involucrata, embryo, regression analysis, uniform design
