摘要
用羧甲基茯苓多糖（ＣＭＰ）预处理人外周血淋巴细胞（ＨＰＢＬ），在以ＰＨＡ和ＣｏｎＡ为主的复合诱生剂诱导培养２４、３６、４８、７２ｈ采样检测的促诱生组ＴＮＦ－α、ＩＬ－２、ＩＬ－６、ＩＦＮγ效价（ｘ±ｓ）分别为４５６．１±２４．２，４７５．６±１８５．７，７４３３±１１８９．９，５５８３．０±５３５．５ＩＵ／ｍｌ，分别比无ＣＭＰ的常规诱生组效价高１．５，３．８，１．８，１．４倍；在以ＮＤＶ－Ｆ病毒为诱生剂诱导培养２２ｈ采样，并经酸化和中性化后检测的ＩＦＮ－α效价可达２１２７４．２±５５２３．０ＩＵ／ｍｌ，比无ＣＭＰ的常规诱生组高１．２倍，均有显著差异（Ｐ＜０．０１），说明ＣＭＰ对上述五种细胞因子均有明显的促诱生效应。此外，还采用ＣＭＰ促诱生技术，对ＴＮＦ－α、ＩＬ－２、ＩＬ－６、ＩＦＮγ和ＩＦＮ－α细胞因子进行了中试，提出了低成本、高效益的中试工艺路线。
The titers of TNFα,IL2,IL6 and IFNγ in HPBL culture, supernatants which were harvested after HPBL were pretreated with CMP and stimulated with PHA and ConA in 24,36 and 72 hours were detected as 456.1±24.2,475.6±185.7,7432±1189.9,5583.0±535.5 IU/ml respectively, which were 1.5,3.8,1.8,1.4 times higher than that of classical induction groups (CMPfree). The titers of IFNα from HPBL cultures induced by NDV for 22 hours were 21274.2±5523.0IU/ml, 1.2 times higher than that of classical induction groups. The finding inducates that CMP can induce TNFα,IL2,IL6,IFNγ/α expression. By using the technique of stimula ting induction with CMP, we prepared the TNFα,IL2,IL6,IFNγ and IFNα intermediate forms. The cytokine intermediate technique provided in the present study is lowexpenditure, high effectiveness, and used widely to produce native cytokines in cellengineering.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1998年第3期194-197,共4页
Immunological Journal
关键词
羧甲基
茯苓多糖
促诱生
细胞因子
中间体
Carboxymethyl pachyman(CMP), Stimulating induction, Cytokines, Intermediate forms
