摘要
目的探讨树突状细胞（DC）对细胞因子诱导的杀伤细胞（CIK）增殖能力、免疫表型、分泌细胞因子水平以及抗白血病细胞作用的影响。方法健康人外周血单个核细胞诱导Dc和CIK细胞，将DC与CIK共培养，以CIK细胞单独培养为对照。用台盼蓝活细胞计数计算细胞扩增倍数，MTT法测定杀伤活性，流式细胞术分析免疫表型，ELISA双抗体夹心法检测分泌干扰素γ（IFN-γ）、白细胞介素12（1L-12）的水平。结果DC—CIK细胞增殖能力明显高于CIK细胞（P〈0．05）；DC，CIK细胞共培养后，CD3＋CD8＋，CD3＋CD56＋阳性细胞比率较同条件下CIK细胞组显著增多（P〈0．05）；共培养3d，DC—CIK细胞上清液中IL-12，INF--／分泌量均比CIK细胞单独培养的分泌量高（P〈0．01，P〈0．05）；在5：1～40：1的效靶比范围内，DC—CIK细胞对白血病细胞的杀伤率显著高于CIK细胞（P〈0．05），且杀伤率与效靶比呈正相关。结论DC—CIK细胞增殖能力和抗白血病活性显著高于CIK细胞，DC—CIK细胞可作为-种临床有效的抗白血病免疫治疗策略。
Objective The purpose was to investigate the effect of dendritic cells （DC） on proliferation,phenotype,secretory cytokines and anti-tumor activity against leukemia cells of cytokine-induced killer cells （CIK）. Methods DC and CIK were prepared from healthy human peripheral blood mononuclear cells. They were co-cultured. CIK cells were cultured alone as control. Increase dnumber of cells were counted by tapanblue staining,killing activity was detected by MTT assay, cells phenotypes were analyzed by flow cytometry,secretions of INF-γ,IL-12 were determined by ELISA. Results The proliferation activity of DC-CIK cells was significantly higher than that of CIK cells （P〈0.05）. Under the same condition, the rate of CD3＋CD56＋ and CD3＋CD8＋ double positive cells in CIK cells were significantly enhanced by co-cultured with DC （P〈0.05）. In DC-CIK cells,the level of IL-12,INF-γ in culture supernatants were all increased noticeably on day 3 compared to CIK cells which were cultured alone （P〈0. 01 ,P%O. 05）. At the effecttor target ratio from 5：1 to 40：1,the ant-tumor effect of DC-CIK cells was much higher than that of CIK cells （P〈0. 05）,and this effect was positive related with the effector target ratio. Conclusion The proliferation activity,anti-tumor effects against leukemia cells of DG-CIK cells were significantly higher than that of CIK cells. DC-CIK is promising as an immunotherapeutic strategy for patients leukemia.
出处
《现代检验医学杂志》
CAS
2009年第4期32-35,共4页
Journal of Modern Laboratory Medicine
