摘要
目的探讨基质金属蛋白酶母（MMP-9）及其抑制剂-1（TIMP-1）在重度慢性阻塞性肺疾病急性加重期（AECOPD）的变化及其临床意义。方法应用酶联免疫吸附试验（ELISA）检测35例健康者（对照组）、40例COPD稳定期和急性加重期患者的血清MMP-9和T1MP-1水平，并分析其与肺功能的关系。结果稳定期COPD组MMP-9高于对照组，但两组MMP-9比较差异无统计学意义（P〉0．05），TIMP-1、MMP-9／TIMP-1均高于对照组（P均〈0．05）；AECOPD组的血清MMP-9、TIMP-1、MMP-9／TIMP-1均高于对照组（分别为P〈0．01，P〈0．05，P〈0．05）；与稳定期比较，AECOPD组MMP-9、TIMP-1、MMP-9／TIMP-1升高（均P〈0．05）；AECOPD组血清MMP-9、TIMP-1、MMP-9／TIMP-1与FEV。％占正常预计值均呈负相关（P均〈0．05），与稳定期组比较，AECOPD组FEV1、FEV1％占正常预计值有明显下降（P均〈0．05）。结论AECOPD患者血清MMP-9、TIMP-1和MMP-9／TIMP-1比值增加，均与FEV，％占正常预计值呈负相关；MMP-9／TIMP-1比例失衡，可能是导致重度AECOPD肺功能下降的机制之一。
Objective To explore the role of matrix metalloproteinase 9 （MMP-9）and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） in acute exacerbation of severe chronic obstructive puhnouary diseases（AECOPD）. Methods The serum levels of MMP-9 and TIMP-1 in 35 healthy volunteers （group A） ,40 severe stable COPD patients （ group B） and paired AECOPD patients （ group C ） were measured by Enzyme-linked Immunosorbent Assay （ELISA）. The lung functions were evaluated also. Results Com- pared with group A, the serum levels of MMP-9 in group B were not statistically higher （P 〉 0.05 ） ,but the serum levels of TIMP-1 ,the ratio of MMP-9/TIMP-1 were statistically higher（ P 〈 0.05 ） ; Compared with group A,the serum levels of MMP-9,TIMP-I and the ratio of MMP-9/TIMP-1 in group C were statistically higher（P 〈 0.01 ,P 〈 0.05 ,P 〈 0.05 ） ;Compared with group B, The serum levels of MMP-9,TIMP-1 ,ratio of MMP-9/TIMP-1 in group C were increased significantly （P 〈 0.05 ） ;2 There was a significant inverse correlation between the levels of MMP-9, TIMP-1 in serum and FEV1% , as well as the ratio of MMP-9/ TIMP-1 （ P 〈 0.05 ）. Compared with group B, FEV1 ,FEV1 % in group C were decreased significantly（ P 〈 0.05）. Conclusions There were significant elevations of serum levels of MMP-9,TIMP-1 and the ratio of MMP-9/TIMP-1 in patients with AECOPD, and all these were inversly correlates with FEV1% ;In severe AECOPD,the imbalance of MMP-9/TIMP-1. maybe contribute to decreased lung function.
出处
《临床内科杂志》
CAS
2009年第8期560-562,共3页
Journal of Clinical Internal Medicine
基金
广东省佛山市科技攻关项目（200608018）
关键词
基质金属蛋白酶
基质金属蛋白酶组织抑制剂
慢性阻塞性肺疾病
Matrix metalloproteinases
Tissue inhibitor of metalloproteinases
Pulmonary disease, chronic obstructive
