摘要
在筛选出产海藻糖合酶菌株SH-110基础上研究其培养特性和最佳的发酵条件。优化培养基组成为:1%麦芽糖、0.5%蛋白胨、0.2%牛肉膏、0.1%K2HPO4、0.12%NaH2PO4。其最佳的发酵条件为:最佳发酵温度为36℃,最佳发酵pH值为7.2,选取最佳装液量为15 mL,发酵终点确定为48h。
The optimal fermentation conditions of Bacterium SH - 110 produceing trehalose synthetase were investigated in this paper. The optimal culture medium were as followed： maltose concentration 1%, peptone 0. 5 %, yeast extraction 0. 2%, K2 HPO4 0. 1% and NaH2 PO4 0. 12%. The optimal environmental conditions were as followed： flask volume 150 ml in 500ml Erlenmeyer - flask, initial pH 7. 2, fermentation temperature 36℃ and fermentation time 48 h.
出处
《中国酿造》
CAS
北大核心
2009年第8期73-76,共4页
China Brewing
关键词
海藻糖
海藻糖合酶
培养特性
发酵条件
trehalose
trehalose synthetase
cultivation property
fermenting conditions
