摘要
目的:探讨小鼠胚胎神经干细胞体外分离培养增殖及分化,为进一步的实验研究提供基础。方法:分离孕13～15d的小鼠胚胎脑组织,在加入碱性成纤维细胞生长因子(bFGF)和表皮细胞生长因子(EGF)的B27无血清培养基中克隆培养,并用10%的胎牛血清诱导其贴壁分化,应用免疫荧光染色方法行巢蛋白(Nestin),β-微管蛋白(β-tublin),胶质纤维酸性蛋白(GFAP)染色,对神经干细胞(NSCs)及其分化的细胞进行鉴定。结果:分离培养的细胞可形成典型的神经球,并传代。细胞表达神经巢蛋白,并可诱导分化为神经细胞和神经胶质细胞。结论:小鼠胚胎神经干细胞能够在体外适宜的条件下大量增殖,并且具有多向分化潜能。
Objective： To isolate,culture and identify the neural stem cells of mouse embryo in vitro for the further related research. Methods ：Brain tissues were isolated from embryonic（E13-15d） mouse, and were cultured with bFGF and EGF in serum-free medium （SFM）. Meanwhile,we induced neural stem cells differentiation and identified it with measure of immunofluorescence including Nestin,β-tublin, GFAP. Results： The cultured ceils could form typical neurospheres and survive well after passaged. Meanwhile, these cells showed nestin-positive and could express specific antigens of neurons, astrocytes and oligodendrocyte after differentiation. Conclusion ： the neural stem ceils derived from mouse embryonic brains are able to proliferate and multiplepotent for differentiation.
出处
《陕西医学杂志》
CAS
2009年第8期939-941,共3页
Shaanxi Medical Journal
基金
广西科技厅资助项目(桂科攻0592007-1G)
关键词
神经干细胞
培养
分化
小鼠
Neural stem cell Culture Differentiation Mouse
