摘要
目的:研究细胞因子信号抑制因子SOCS1、SOCS2、SOCS3基因在正常人早孕蜕膜基质细胞的表达及其意义。方法:体外分离培养人早孕蜕膜基质细胞,采用逆转录聚合酶链反应(RT-PCR)及Western blotting法检测SOCS表达,酶联免疫吸附剂测定(ELISA)法检测细胞IL-10、IFN-γ的分泌。结果:无血清培养蜕膜基质细胞未见SOCS1表达,但表达SOCS2、SOCS3;细胞培养14h所分泌的IL-10高于细胞培养2h时的水平(P<0.05),IFN-γ分泌则无明显变化(P>0.05);IL-10(0.1ng/ml)作用蜕膜基质细胞14h内,不同程度上调SOCS2、SOCS3表达。结论:人早孕蜕膜基质细胞自分泌、旁分泌的IL-10可能诱导细胞SOCS2、SOCS3的表达。
Objective： To explore the expression and significance of suppressors of cytokine signaling （SOCS） l, SOCS2, SOCS3 in human decidual stromal cells （DSCs） during first-trimester. Methods： The human DSCs were isolated and cultured in vitro for 48 h, and were serum-starved overnight, then treated with IL-10 for 14 h. Semi-quantitative RT-PCR and Western blotting were used to detect the SOCS 1, SOCS2, SOCS3 mRNA and protein expression. IL-10 and IFN-γ
secretion were detected by enzyme linked immunosorbent assay （ELISA）. Results： The expression of SOCS 1 was not detected in DSCs under serum-starved condition forl4 h, but the low expression of SOCS2 and SOCS3 was founded. The level of IL-10 was increased significantly at 14 h compared with that of 2 h under serum-starved condition （P〈0.05）, and IFN-γ maintained low level within 14 h compared with the secretion of 2 h, P〉0.05. The SOCS2 and SOCS3 mRNA and protein expressions were significantly increased in decidual stromal cells response to IL-10 （0.1 ng/ml） respectively. Conclusion： IL-10 may be the one of cause inducing SOCS2 and SOCS3 expression, which autosecreted and parasecreted by DSC during first-trimester.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2009年第7期432-437,共6页
Reproduction and Contraception
基金
国家自然科学基金项目(30572446)
上海市科委现代生物与制药基金项目(02D219115)
