摘要
为观察人单纯疱疹Ⅱ型病毒感染对培养的牛主动脉平滑机细胞形态及合成胶原的影响，用50％组织培养感染量的病毒悬液于37℃感染培养的牛主动脉平滑肌细胞，于倒置显微镜和电镜下观察平滑肌细胞形态改变，以3H－脯氨酸掺入平滑肌细胞合成的3H－羟脯氨酸的量作为测定胶原的指标。结果发现，感染24h后，平滑肌细胞逐渐出现细胞变圆、肿胀和局部坏死脱落等形态改变；感染24、36和48h后平滑肌细胞胶原的含量与对照组相比明显降低。提示人单纯疱疹Ⅱ型病毒可能作为一种局部损害因子，抑制血管平滑肌细胞胶原合成，损害中滑肌细胞，可参与动脉粥样硬化的发病过程。
Aim: To investigate the possible role of HSV-Ⅱ in the etiology of atherosclerosis, the effects of herpes simplex virus type Ⅱ (HSV-Ⅱ ) infection on morphology and collagen synthesis of smooth muscle cells (SMC) were observed.Methods: Bovine aortic SMCs in culture were infected with HSV- Ⅱ, using 3H-proline incorporating into 3H-hydroxyproline by SMC as a criterion of collagen synthesis in SMC.Results: The significant cytopathic effect (CPE) such as cell rounding, swallowing, focal necrosis and detachrnent was observed 24 h after infection; there was a significant decrease in the synthesis of collagen in bovine SMC at 24 h, 36 h and 48 h after infection compared to controls.Conclusion: The results suggested that HSV-Ⅱmay be one of the injurious agents of inciting atherosclerosis by inhibiting collagen synthesis in SMC as well as injury factor for SMC.
出处
《中国动脉硬化杂志》
CAS
CSCD
1998年第2期102-104,共3页
Chinese Journal of Arteriosclerosis
基金
浙江省卫生厅资助
关键词
血管平滑肌细胞
胶原
动脉粥样硬化
疱疹病毒
Herpes simplex virus type Ⅱ Smooth muscle cell
Collagen
Atherosclerosis
