摘要
用鹅副粘病毒WF01G分离株进行9-10日龄SPF鸡胚尿囊腔接种,成功增殖了该病毒,采用一步法RT-PCR技术扩增WF01G病毒的F基因,获得了1条长约1.7 kb的特异性条带。对PCR扩增产物测序。结果表明,扩增片段大小为1782 bp,含有1个1662 bp的开放性阅读框,编码554个氨基酸。核苷酸同源性分析表明：WF01G株与其他7株鹅副粘病毒的同源性为84.8%-98.8%,与国内外其他NDV F基因的同源性为84.7%-93.8%,其中与国内标准强毒株F48E9的同源性为86.8%,说明WF01G与国内外的传统毒株有较大变异。与Tai-wan95株的同源性为93.8%,说明WF01G与Taiwan95株亲缘关系较近,具有较高的相似性。F蛋白裂解位点的氨基酸顺序为112Arg-Arg-G ln-Lys-Arg-Phe117,表明为副粘病毒强毒株。蛋白疏水性和抗原性分析表明与标准强毒F48E9株相比没有太大的变异。
The paramyxovirus of WF01G was grown in lO-day-old SPF embrocated eggs. The F gene of Fengyang strain WF01 G of goose was amplified by one step RT-PCR. The sequencing analysis showed that the sequence of the F gene was 1782 bp and encoded a protein of 554 amino acids. The homology between WF01G and the other goose paramyxovirus strains was 84.8% to 98.8%. The homology between WF01G and the NDV strains isolate inside and outside China was from 84.7% to 93.8%. The WF01G F gene＇s nucleotide sequence shared 86.8% homology with that of the NDV strain F48E9. The ORF of WF01 G F gene shared 93.8% nucleotide homology with that of the NDV strain Taiwan95. The results indicated the mutation of the F gene had varied largely, compared with classic NDV. The deduced amino acid sequences near clear-age site of F0 proteins showed a 112Arg-Arg-Gln-Lys-Arg-Phe117 motif, eomforming well with that of the virulent strains. The analysis of WF01 G F protein hydrophobieity and epitope showed that W-F01 G strain was not a variation.
出处
《中国微生态学杂志》
CAS
CSCD
2009年第7期581-584,共4页
Chinese Journal of Microecology
基金
国家自然科学基金(30671556)
安徽省教育厅自然科学基金一般项目(KJ2007B298)共同资助
关键词
鹅副粘病毒
F基因
序列测定
病毒毒力
同源性分析
Newcastle disease virus from goose
F gene
Gene sequencing
Virulence of NDV
Analysis of nucleotide homology
