摘要
本文报告采用恙虫病立克次体表面蛋白５６ＫＤａ基因编码区构建的群、型特异引物，ＰＣＲ检测１４份恙虫病患者血提取的ＤＮＡ模板，扩增后均见有３１７～３３２ｂｐ片段的扩增带。与血清学检测比较，ＰＣＲ具有敏感、早期诊断的意义。群引物扩增的阳性产物再经ＰＣＲ分型，结果证明山东蒙阴地区存在Ｋａｗａｓａｋｉ型Ｒｔ。
olymerase chain reaction(PCR) method was used for detecting and typing rickettsia tsutsugamushi(RT).The primers of the group and type were derived from the typespecific antigen gene DNA sequences(encodes the 56kPa antigen) of Rt specific DNA band of 317￣332 bp were observed by PCR in all the DNA extracted from the blood of 14 petients,it is sensitive and early for diagnosis than serum deticting.The amplification products with group primers was typed,the Rt is blong to kowvasali in Mengyin,Shandong province.
出处
《现代预防医学》
CAS
1998年第2期156-157,共2页
Modern Preventive Medicine
