摘要
目的了解慢性肝病患者肝组织中庚型肝炎病毒（ＨＧＶ）的存在状况。方法采用免疫组织化学方法，用抗ＨＧＶＮＳ５单克隆抗体对１１０例血清学病毒标记非甲非戊、乙型及丙型肝炎的慢性肝病患者肝组织中的ＨＧＶ抗原进行了检测。结果ＨＧＶ的总检出率为３２７％（３６／１１０），非甲非戊、乙型及丙型肝炎病毒的检出率分别为２１％（４／１９）、３６％（２５／６９）及３２％（７／２２）；活动性肝硬化、原发性肝癌及慢性重型肝炎中ＨＧＶ的检出率为２２％（１０／４５）、４３％（２０／４７）及３３％（６／１８），不同血清病毒标志组或病理类型之间其检出率差异均无显著意义（χ２＝４３１，１．５２；Ｐ＞０１０，Ｐ＞０２５）。ＨＧＶＮＳ５Ａｇ主要于肝细胞或癌细胞胞浆内表达，阳性肝细胞呈弥漫或大片灶状分布，免疫组织化学双标记染色显示ＨＢｓＡｇ或丙型肝炎（ＨＣＶ）ＮＳ３Ａｇ阳性细胞呈单个或小片灶状，多与ＨＧＡｇ阳性细胞位于同一区域内，或在同一肝细胞内表达。
Objective To investigate the existence of hepatitis G virus (HGV) in liver tissue. Methods HGV NS5 antigen was detected by immunohistochemical method in paraffinembeded liver tissue of autopsy patients with chronic liver disease. Results Among 110 samples, 32.7% (36/110) had been detected out HGAg in their liver. When serologica marker was used,the detection rate was 21% (4/19) in HNAE, 36% (25/69) in HBV and 32%(7/22) in HCV infectious group, respectively. HGAg expression in hepatocytes was also found pathologically in 22% of 45 patients with active cirrhosis, 43% of 47 patients with hepatocellular carcinoma, and 33% of 18 patients with chronic fulminent hepatitis. The staining signal of HGV NS5 antigen was mainly located in the cytoplasm of liver or neoplasm cells, and the positive cells were distributed diffusely in pseudolobule or liver tissues. Conclusion The infection of viral G is often seen in liver tissue of patients with chronic liver disease.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第8期598-600,共3页
National Medical Journal of China
