摘要
目的研究拉西地平对异丙肾上腺素(Iso)、佛司可林和美兰(MB)增强心肌细胞收缩、舒张功能的影响。方法采用IonOptix单细胞动缘检测系统,测定心肌细胞收缩幅度和收缩、舒张速度等,指标均由计算机自动实时采集并记录。结果Iso、Fors-kolin和MB增强单个心肌细胞收缩幅度(ph)和单个心肌细胞收缩幅度/单个心肌细胞长度百分比(ph/bl),最大缩短速率(+dL/dt)和最大复长速率(-dL/dt),上述指标的增强均有显著性差异(P<0.01)。与对照组比较,Iso(10.0nmol/L)能使ph、ph/bl、+dL/dt和-dL/dt分别增加40%、41%、60%和50%。Forskolin(1.0μmol/L)能使ph、ph/bl、+dL/dt和-dL/dt分别增加89%、120%、106%、86%;用MB(10μmol/L)后,ph、ph/bl、+dL/dt和-dL/dt分别为0.15±0.03μm、12%±4%、2.3±0.4μm/s和2.4±0.5μm/s,用药前后差异有统计学意义(P<0.01)。3.0μmol/L的Larcidipine能抑制Iso引起ph、ph/bl、+dL/dt和-dL/dt的升高(P均<0.05),Lar-cidipine能同时降低Forskolin和MB引起ph、ph/bl、+dL/dt和-dL/dt的增加。结论Iso、Forskolin和MB使心肌细胞收缩和舒张功能均增强;Larcidipine可抑制Iso、Forskolin和MB的作用。
Objective To investigate the inhibitive effect and the mechanism of larcidipine on the accentuated myocardial function of systole and diastole enhanced by isoproterenol （Iso）, forskolin and MB. Methods The systolic amplitude was measured with a video edge tracker method. The following parameters were recorded by computer： ph （peak height）, peak height/baseline percent （ph/bl）, maximal velocity of systole （＋dL/dt） and maximal velocity of diastole （-dL/dt）. Results Iso, forskolin and MB increased electrically-induced systole including ph, ph/bl, ＋dL/dt and -dL/dt. Iso, forskolin and MB augmented the myocardial function of systole and diastole in a significant sense. Iso in a concentration of 10. 0nmol/L augmented electrically-induced systole and ph, ph/bl, ＋dL/dt and -dL/dt, and these parameters increased by 40%, 41 %, 60% and 50%, respectively. Compared with the control group, forskolin in a concentration of 10. 0pmol/L increased ph from （0. 12±0. 03μm to 0. 25±0. 06μm, ph/bl from 12±3% to 28±6%, ＋dL/dt from 1.8±0. 5μm/s to 3. 7±0.8μm/s, -dL/dt from 1. 8±0. 2μm/s to 3. 6±0. 6μm/s, and these indexes increased by 89%, 120%, 106% and 86%, respec tively. MB in a concentration of 10μmol/L also significantly increased the ph, ph/bl, ＋dL/dt and --dL/dt to 0. 15±0. 03μm, 12%± 4%, 2. 3±0. 4μm/s and 2.4±0. 5μm/s, respectively. Lareidipine in a concentration of 3.0μmol/L significantly decreased the parameters of ＋dL/dt and -dL/dt, which were increased by Iso, forskolin and MB. Conclusions Iso, forskolin and MB may increase the myocardial function of systole and diastole. These drugs might enrich the intracellular calcium indirectly and directly. The effects of Iso, forskolin and MB might be attenuated by Larcidipine. Lareidipine may not only block the dihydropyridine receptor, but also inhibit the other calcium influx.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2009年第8期997-999,1004,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助项目(30870902)
