摘要
本研究设计不同的免疫程序,用禽流感(H5+H9)二价灭活疫苗(H5N1Re-1+H9N2Re-2株)免疫黄羽种鸡,通过对H5亚型和H9亚型禽流感HI抗体滴度监测,探讨H5亚型和H9亚型禽流感HI抗体消长规律及禽流感(H5+H9)二价灭活疫苗的免疫程序。结果表明,用禽流感二价灭活疫苗免疫黄羽种鸡后,H5亚型和H9亚型禽流感HI抗体的消长规律基本同步,其抗体滴度阶段性峰值出现在免疫后的第21～28天。根据试验结果,建议用禽流感二价灭活疫苗免疫黄羽种鸡的免疫程序设为:首免(14d),0.3mL/只;二免(56d),0.5mL/只;三免(119d或开产前4周),0.5mL/只。
To study the protective efficacy and the optimal immunization procedure of the H5＋H9 bi- valent inactivated vaccine （H5N1 Re-1 and HgN2 Re-2） in the breeder of Yellow Feather chickens, the bi- valent vaccine immunized Yellow Feather chickens by different immunization procedure. Results showed that the vaccinated chickens have the similar antibody variation to H5 and H9 but antibody titer of H9 he- magglutinin-inhibition （HI） is little higher than its H5 HI. The HI antibodies against H5 and H9 became detectable and increased at 1 to 2 week post-vaccination （p. v. ） and reached a peak about 3 to 4 weeks p. v. After the secondary and third immunization, HI antibody titers quickly ascended and reached 101og2 at 4 weeks after the third immunization. After the secondary immunization, values of the dispersion of HI anti- body changed little. The breeding chickens were vaccinated third before egg-laying. The first immunization of these chickens is at the 14 day-old and injected with 0.3 mL per chicken. The secondary immunization is at the 56 day-old and injected 0.5 mL per chicken. The third immunization is at the 119 day-old or before egg-laying.
出处
《中国兽医杂志》
CAS
北大核心
2009年第7期14-16,共3页
Chinese Journal of Veterinary Medicine
基金
国家科技支撑计划(2006BAD06A16-2)
教育部创新团队项目(IRT0723)
教育部新世纪优秀人才支持计划(NCET-06-0752)
广东省自然科学基金创新团队项目(5200638)
广东省科技计划项目(2005A20901003
2006B21101001
2006B0152)
广东省自然科学基金重点项目(06105471)
关键词
禽流感
H5+H9亚型
二价灭活疫苗
免疫程序
种鸡
avian influenza
H5 and H9 subtype
bivalent inactivated vaccine
immunization program
breeder
