摘要
应用免疫组织化学SABC法检测第2,3,4三个月胎龄段,Cx43和Pax3蛋白在人胚胎食管组织中的表达.结果表明:第2个月胚龄时,Cx43和Pax3在食管肌层肌细胞中呈阳性表达,在肌间神经丛处神经细胞呈阴性表达;第3个月胎龄段,Cx43和Pa3在食管肌层肌细胞阳性表达与第2个月胚龄相似,在肌间神经丛处大部分神经细胞呈阳性表达;第4个月胎龄段,Cx43和Pax3在食管肌层神经细胞和肌细胞均呈弱阳性表达.因此,Cx43和Pax3蛋白与人胚胎早期食管肌层组织细胞的生长发育关系密切.
The objective of the paper is to explore the patterns of Connexin43 （Cx43） and Pairedbox3 （Pa.x3） protein expressions in the esophageal muscular layers of a human embryo. The SABC method in Immunohistochemistry has been employed to examine the expressions of Cx43 and Pax3 proteins in the muscular layers of the esophagus in human em- bryos from the second to fourth months of gestation.The following are the results ： In the second month of gestation, their muscle cells of the esophageal muscular layers are positive while the nerve cells in the myenterie plexus are negative; In the third month, the Cx43 and Pax3 expressions in the muscle cells in the esophageal muscular layers are similar to those of the second month, with most nerve cells positive in the myenteric plexus ; In the fourth month, the Cx43 and Pax3 protein expressions are weakly positive both in the muscle cells and nerve cells of the esophageal muscular layers. A conclusion is reached：Their Cx43 and Pax3 proteins are closely related to the growth and development of the cells and tissues in the esophageal muscular layers in human embryos.
出处
《绍兴文理学院学报》
2009年第8期78-80,共3页
Journal of Shaoxing University
