摘要
目的:分析西安地区耐利福平(RIF)结核杆菌临床分离株rpoB基因突变的特点,探讨快速检测结核杆菌耐RIF基因型的方法.方法:对120株西安地区收集的结核杆菌临床分离株(药敏结果为耐RIF81株,对RIF敏感39株),分别采用聚合酶链反应-单链构象多态性(PCR-SSCP)和等位基因特异性多重聚合酶链反应(MAS-PCR)检测rpoB基因突变情况.结果:PCR-SSCP检测,耐RIF菌株中rpoB基因突变发生率为85%(69/81),39株敏感株中仅有1株发生突变,突变率为2%(1/39).MAS-PCR检测,耐RIF菌株中rpoB基因突变发生率为93%(75/81),39株敏感株中未发现rpoB基因突变;531,516和526位点突变的比例分别为45%(34/75),24%(18/75)和20%(15/75).结论:西安地区结核杆菌临床分离株耐RIF与rpoB基因突变相关,MAS-PCR检测rpoB基因可作为临床耐RIF结核杆菌的简单快速准确的早期诊断方法.
AIM： To analyze the character of rpoB gene mutation in rifampin （ RIF ） -resistant Mycobacterium tuberculosis （M. TB） strains isolated from tuberculosis patients in Xi＇an and to develop and evaluate methods to detect the R/F-resistant gene of M. TB. METHODS： RpoB gene mutation was examined in 120 M. TB strains （81 RIF-resistant and 39 RIF-sensitive） isolated from tuberculosis patients in Xi＇an by polymerase chain reactionsingle strand conformation polymorphism （PCR-SSCP） and multiplex allele specific polymerase chain reaction （MAS-PCR）. RESULTS： By PCR-SSCP, the ratio of rpoB mutation in RIFresistant M. TB strains was 85% （69/81） and there was only 1 strain with rpoB mutation in the 39 RIF-sensitive M. TB strains. By MAS-PCR, the ratio of rpoB gene mutation in RIF-resistant M. TB strains was 93% （75/81） and no rpoB gene mutation was found in the 39 RIF-sensitive M. TB strains. The ratio of rpoB gene mutation point at eodon 531, eodon 516 and codon 526 was respectively 45% （ 34/75 ）, 24% （ 18/75 ） and 20% （ 15/75 ）. CONCLUSION： RIF resistance of M. TB strains isolated from tuberculosis patients in Xi＇an is correlative with rpoB gene mutation. Detection of rpoB gene using MAS-PCR can be a simple, rapid and accurate method for the early diagnosis of RIF-resistant M. TB.
出处
《第四军医大学学报》
CAS
北大核心
2009年第15期1360-1362,共3页
Journal of the Fourth Military Medical University
基金
西安市科技支撑计划基金(YF075158)
