摘要
目的:观察紫杉醇及环氧化酶-2(COX-2)选择性抑制剂塞来昔布(Celecoxib)对人胃腺癌细胞株BGC-823COX-2及P-糖蛋白(P-gp)表达的影响,探讨COX-2在化疗药物诱发多药耐药(MDR)产生机制中的作用。方法:采用MTT法检测紫杉醇不同剂量、不同时间点及塞来昔布不同剂量对胃腺癌细胞株BGC-823生长的影响,在此基础上采用Western blot方法检测一定剂量范围内某一时间点的紫杉醇对BGC-823 COX-2、P-gp表达的影响及联合塞来昔布后两种蛋白表达的变化。结果:紫杉醇对胃腺癌细胞株BGC-823的生长为细胞毒作用,呈时间和剂量依赖性。在一定剂量范围内和某一时间点,随着紫杉醇剂量逐渐升高,BGC-823的COX-2、P-gp表达均呈上升趋势;且这两种蛋白在联合塞来昔布应用后表达均呈下降趋势。结论:紫杉醇可诱导胃腺癌细胞株BGC-823 COX-2及P-gp蛋白的表达,这种表达能被塞来昔布所抑制。COX-2表达的诱导在紫杉醇诱导的P-gp表达中起着重要作用。
Objective：To observe the effect of paclitaxel and a selective cyclooxygenase-2（COX-2） inhibitor named celecoxib on the expression of COX-2 and P-giycoprotein（P-gp） in human gastic adenocarcinoma ceil line BGC823, and to study the role that COX-2 played in the mechanism of chemotherapy-induced multi-drug resistance（MDR）. Methods：The effects of paclitaxel with vari- ous doses and time points on BGC823 cell growth was assessed by MTT assay, and so did the effects of celecoxih with different doses. The effect of a dose-ranging paclitaxel in at one time point and the change of combining celecoxib with paclitaxel on the COX-2 and P-gp protein expression was detected by Western blot. Results：Paelitaxel had the effect of cellular toxicity on BGC823 growth, which in dose-dependent and time-dependent manners. With a limited ranging dose and at certain time piont, when the dose of paclitaxel increasing gradually, the expression of both COX-2 and P-gp showed rising trend in BCC823 cell line. And the expression of the two proteins could indicate a downtrend after combined with celecoxib. Conclusion：Paclltaxel can induce the expression of COX-2 and P-gp increased in BGC823 at the same time, and the enhanced expression could be inhibited by celeeoxib. The inducing of COX-2 expression should take a important part in paclitaxel-induced expression of P-gp.
出处
《临床肿瘤学杂志》
CAS
2009年第7期602-605,共4页
Chinese Clinical Oncology
