摘要
目的探讨雌激素对血管平滑肌细胞(VSMC)增殖的双重效应机制。方法采用Westernblot、电镜形态定量及细胞计数的方法,动态检测原代培养大鼠VSMC在有或无10-8mol/L17β-雌二醇(E2)存在下,雌激素受体(ER)α和β表达变化与细胞表型转变及增殖时相的关系。结果无E2存在时,VSMC在从收缩型向合成型转变(原代培养第0到5天)及活跃增殖(第5到12天)过程中,ERβ表达无明显变化,但ERα表达明显上升,导致ERα/ERβ比值升高。这种变化并不随VSMC表型的恢复及增殖停止而逆转。有E2存在时,ERα/ERβ比值在第5天时低于对照组,而第9天后各时点均高于对照组;这种影响与E2对不同状态VSMC的不同作用基本对应,即延长原代收缩型SMC的增殖潜伏期,但促进已发生表型转变的VSMC增殖。结论雌激素对不同表型VSMC的双重效应与表型转变前后ERα/ERβ比值变化有关。
Objective To investigate the mechanisms underlying the dual effects of estrogen on proliferation of vascular smooth muscle cells （VSMC） . Methods The expression of estrogen receptors （ER） α and β, and its relationship with the phenotypic modulation and cellular proliferation were investigated in the primary cultured female rat VSMCs using Western blot, quantitative electronmicroscopy and cell counting. Results When the VSMCs underwent the phenotypic modulation from a contractile to a synthetic phenotype and intensive proliferation, their expression of ERβ did not change obviously; but the expression of ERα increased significantly, leading to an elevation of the ERα/ERβ ratio. These changes did not reverse after the restoration of the phenotype and the case of the proliferation of the cells. Administration of 10^-8 mol/L 17β-estradiol （E2） suppressed the increased expression of ERa in the cells during the phenotypic modulation, but up-regulated the ERα expression in the modulated cells. This action was in line with the dual effects of the hormone on the cells, that is, elongating the proliferative latency of the contractile VSMC but accelerating the proliferation of the modulated VSMC. Conclusion The dual effects of estrogen on proliferation of VSMC in different phenotypes are related with the changes of the ERα/ERβ ratio during the cell phenotypic modulation.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2009年第4期392-395,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金资助项目(30470906)
中澳科技合作特别基金资助项目(30711120185)
关键词
雌激素受体
平滑肌细胞
表型转变
细胞增殖
Estrogen receptor
Smooth muscle cells
Phenotypic modulation
Cell proliferation
