摘要
目的:探讨帕金森(PD)大鼠模型体内脑黑质和纹状体内骨形成蛋白4(BMP4)及其mRNA表达的变化规律。方法:用六羟基多巴胺(6-OHDA)建立PD模型后,第2、4、6、8和10周时处死大鼠,取左侧黑质、纹状体,用免疫组化、酶联免疫、荧光定量PCR技术从蛋白水平和基因水平检测BMP4及其mRNA表达。结果:BMP4及其mRNA表达基本一致,其BMP4及mRNA表达均呈双峰,其蛋白表达在纹状体内第2周和6周时达高峰、在黑质内第2周和8周时达高峰,其mRNA表达在纹状体内第2周和8周时达高峰、在黑质内第4周和8周达高峰,差异均有统计学意义(P<0.05)。结论:PD大鼠模型体内,BMP4及其mRNA不呈现稳定的低表达,而是有波动性,明确BMP4及其mRNA处于稳定低表达的时间后,为下一步的治疗实验时间点的选择上奠定了基础。
Objective： To observe the expression of bone morphogenetic protein4 （BMP4） and mRNA of rat model of Parkinson＇s Disease（PD） induced by 6-OHDA with different times. Methods： Animal models of rats with 6-OHDA and different times （2,4,6,8,and 10 weeks） were established.The expressions of BMP4 and BMP4mRNA in substantia nigra and striatum were detected with ELISA and RT-PCR. Results： The expression of BMP4 and BMP4mRNA was similar between substantia nigra and striatum, expressing in double-peak, with statistical significance （P〈0.05）. Conclusions： The low expression of BMP4 and mRNA appears unstable after the estab- lishment of the rat model of PD;however, determination of the stable and low expression of BMP4 and mRNA will be conducive to the choice of the time of therapy for PD.
出处
《现代生物医学进展》
CAS
2009年第13期2410-2412,2439,F0002,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金(NSFC30472272
NSFC30772861)
关键词
帕金森病
骨形成蛋白4
大鼠
Parkinson＇sdisease
Bone morphogenetic protein4
Rat
