摘要
目的探讨鬼针草总黄酮(TFB)的对大鼠免疫性肝纤维化治疗作用及机制。方法SD大鼠随机分为正常组、模型组、TFB大、中、小剂量组和秋水仙碱阳性药对照组,采用腹腔注射猪血清诱导肝纤维化模型。造模后,给药组分别灌服相应的受试药物,正常组和模型组灌服等量的生理盐水。观察大鼠体重的变化及肝脾指数,取血清测定大鼠丙氨酸氨基转氨酶(ALT)、天冬氨酸氨基转移酶(AST)的水平,取肝组织测定羟脯氨酸(Hyp)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,取固定部位肝组织HE染色及Masson染色病理组织学检查。结果TFB能增加大鼠体重,抑制肝纤维化大鼠肝、脾指数上升,显著降低肝纤维化大鼠血清中ALT、AST的水平,降低肝组织Hyp、MDA含量,提高SOD及GSH-Px活性;病理组织学检查结果显示TFB组肝脏组织结构明显改善,肝纤维化程度减轻。结论TFB对猪血清所致大鼠免疫性肝纤维化有明显治疗作用,其机制可能与抗氧化作用有关。
Objective To observe the therapeutic effects and antioxygen mechanism of total flavones of Bidens bipinnata L （TFB） on immunological liver fibrosis in rats. Methods The pig serum was injected into abdominal cavity （0.5 ml, twice a week） for 12 weeks so as to induce hepatic fibrosis, and then the rats were treated with TFB daily for 10 weeks and killed at the 22nd week. The ALT, AST in serum were assessed by UV. The Hyp, MDA, SOD, GSH-Px in liver tissue were observed by UV. Liver samples collected after experiment which were stained with hematoxylin-eosin （HE） staining, Masson staining and scored. Results Compared with model group, TFB increased weight and inhibited the elevation of index of liver and spleen. Furthermore, TFB significantly reduced ALT, AST content in serum. Further study showed that TFB treatment markedly reduced Hyp, MDA in liver tissue, increased SOD, GSH-Px activity. Liver histopathological examination showed TFB could also ameliorate fibrogenesis and pseudolobule formation by HE staining and masson staining. Conclusion TFB significantly reduces pig serum-induced liver fibrosis in rats, probably by its free-radical scavenging ability.
出处
《安徽医科大学学报》
CAS
北大核心
2009年第4期462-465,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省科技攻关重点项目(编号:07010302178)
安徽省自然科学基金项目(编号:090413139)
关键词
肝硬化/中药疗法
大鼠
鬼针草总黄酮
liver cirrhosis/zhong yao liao fa
rats Free word total flavones of Bidens bipirmata L（TFB）
