摘要
目的探讨甲胎蛋白（AFP）、白细胞介素（IL）-18基因修饰树突状细胞（DC）较以往AFP基因修饰DC作为肝癌免疫治疗瘤苗是否更具优势。方法分离外周血单个核细胞定向诱导为DC，以Ad—IL-18和Ad—AFP共感染DC，感染后细胞通过噻唑蓝（MTY）比色法检测其激活的T细胞对HepG2的杀伤活性。结果检测结果显示目的基因能够表达于DC细胞中。流氏细胞仪（FCM）结果显示共感染的DC细胞均高表达CD1a（73．4％）、CD11c（84．3％）、CD80（89．5％）、CD86（87．9％）和HLA—DR（91．7％）。CTL结果显示IL-18／AFP—DC—T对HepG2的杀伤率（67．49±3．24）％明显高于对SMMC7721细胞（27．32±1．75）％和K562细胞（17．31±1．56）％的杀伤率，另外共感染IL-18／AFP—DC—T组对HepG2的杀伤率与AFP—DC—T、IL-18-DC—T组比较，其差异有统计学意义。结论AFP和IL—18基因修饰DC，能够在体外诱导特异性细胞毒性T淋巴细胞（CTL）效应，而且对HepG2细胞杀伤率大于AFP转染DC组。
Objective To investigate the immune functions of dentritic cells （DCs） transduced with Ad-IL-18,Ad-AFP in vitro,compare their antiviral and antitumor effects with those of Ad-IL-18/Ad- AFP DCs and Ad-AFP DCs. Methods Peripheral blood mononuclear cells （PBMC） from HCC patients were isolated by blood separator. PBMC were cultured in vitro for one week to induce DCs. After transfection with Ad-IL-18 and Ad-AFP （IL-18/AFP-DC） ,the stimulated lymphocytes were tested by cytotoxicity capacity assay. Results The results showed that DCs could infect and express relevant genes. The expression of CD11 c （84.3%）, CD1 a （73.4%）, CD80 （ 89.5 % ）, CD86 （ 87.9% ） and HLA-DR （ 91.7% ） on adenovirus transduced DCs were up-regulated. The cytotoxieity mediated by IL-18/AFP-DC-T was higher than that mediated by DC-T and T,and that mediated by IL-18/AFP-DC-T was higher than that mediated by IL-18-DC-T and AFP-DC-T. Conclusion DCs co-infected with these vectors can induce antitumor CTL response in vitro. And Ad-AFP and Ad-IL-18 transfected DCs could induce stronger CTL response against HepG2 than AFP gene-transfected DCs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第8期961-963,共3页
Chinese Journal of Experimental Surgery
关键词
癌
肝细胞
树突状细胞
细胞毒T淋巴细胞
免疫治疗
Carcinoma,hepatocellular
Dendritic cells
Cytotoxic T lymphocytes
Immunotherapy
