摘要
为获得藏灵菇嗜热链球菌Tx菌株胞外多糖(EPS)的纯品,探索了EPS粗品的纯化方法。利用Sepharose CL-6B进行凝胶分级纯化,并对EPS纯品采用紫外全波长扫描、凝胶色谱鉴定及EPS纯度测定。结果表明:最佳纯化条件为:采用0.05～0.06mol/L氯化钠溶液梯度洗脱,流速为0.25ml/min,样品上样浓度为1.0mg/ml,上样量为1.0ml。在最佳纯化条件下得到的EPS纯度为98.01%,是纯化前的1.4倍,为今后进一步研究EPS结构与功能提供了实验基础。
In order to get the pure exopolysaccharides produced by Streptococcus thermophilus Tx from Tibetan Kefir. The purification methods were analyzed, and at last the best purification method was found. Sepharose CL-6B was used to make a classical purification, using ultraviolet scan and chromatogram to identify the purification effect, the purification detection. The results showed that optimization purification conditions： adoption sodium chloride 0.05-0.06 mol/L, the velocity 0.25 ml/min, the sample concentration 1.0 mg/ml, the sample capacity 1.0 ml. On optimization purification conditions EPS purity is 98.01%, 1.4 times larger than former, which provides the basement for the study of EPS structure and function.
出处
《中国农学通报》
CSCD
北大核心
2009年第14期76-80,共5页
Chinese Agricultural Science Bulletin
基金
北京市自然科学基金资助项目"藏灵菇益生菌产生物活性物质及生理功能的研究"(5062006)
农产品加工及贮藏工程北京市重点建设学科资助项目(PXM2009_014207_078172)
关键词
嗜热链球菌
胞外多糖
纯化条件
Streptococcus thermophilus, exopolysaccharides, purification conditions
