摘要
以田间生长的苜蓿为材料进行卡那霉素(Kan)涂抹叶片筛选转基因植株最佳浓度和适宜观察时间的研究,并对筛选的抗性植株进行了PCR检测验证。结果表明,Kan叶片涂抹田间筛选的最佳浓度为1000mg/L,观察时间以12天为宜;经PCR检测验证,Kan筛选抗性苗的阳性检测率达到93.8%。研究建立了一种在田间对转基因苜蓿进行大量筛选的简便方法,对于转基因苜蓿材料的选育具有重要利用价值。
Kanamycin can he used to select the transformed plants containing the npt Ⅱ gene. In order to assay the optimal screening concentration to npt Ⅱ gene, Kanamycin was smeared to the surface of the transformed alfalfa leaves. It showed that 1000 mg/L Kanamycin with 12 days＇ selecting was best. This method was compared with PCR as well, and the results suggested that the equivalent ratio was 93.8 % in the resistant plants. Accordingly, a simple method of filed screening gene transgenic alfalfa has been made. This result is very useful for the transgenic alfalfa to be screened and cultivated.
出处
《中国农学通报》
CSCD
北大核心
2009年第14期22-26,共5页
Chinese Agricultural Science Bulletin
基金
山东省农业良种工程项目"抗旱耐盐苜蓿新品种(系)选育及产业化开发"(2006lz12-02)
山东省科技攻关项目"林木抗逆性基因工程遗传改良研究"(011100104)
关键词
转基因苜蓿
Kan
涂抹
PCR检测
transgenic alfalfa, Kanamycin, smear, plolymerase chain reaction detection
