摘要
目的:观察丹参强化的EN制剂对内毒素诱导S-MODS模型大鼠多器官功能障碍的保护作用。方法:建立大鼠内毒素S-MODS模型。将70只大鼠随机分为正常组(10只)、模型组(20只)、能全素组(20只)和丹参组(20只),实验周期为12 d。实验第1天起正常组和模型组大鼠正常喂养加等渗盐水灌胃,能全素组和丹参组用能全素和丹参EN制剂灌胃。第4天正常组经鼠尾静脉注射等渗盐水1 mL/kg,其他三组经相同部位注射内毒素3mg/kg。第11天停止灌胃。分别于静脉注射内毒素前24 h、后24 h和第9天检测血小板计数。静脉注射内毒素后第9天,检测生化指标和细胞因子水平,同时送检肝、肾、肺和小肠组织病理。结果:大鼠内毒素造模成功。静脉注射内毒素后第9天与注射后24 h相比,丹参组大鼠症状改善,血小板计数恢复,促炎细胞因子IL-6、IL-1β和TNF-α的浓度明显低于模型组和能全素组(P<0.05)。病理观察发现,丹参组大鼠肝、肾、肺和小肠组织损害较其他组轻。结论:丹参强化的EN制剂能保护肠黏膜,减轻S-MODS大鼠全身炎症反应及肝、肾、肺和小肠的损害,降低促炎反应的细胞因子,抑制脓毒症炎症反应。
Objective：To explore the protective effect of salvia miltiorrhiza for enteral nutrition in S- MODS model rats. Methods： 70 Wistar rats were randomly divided into four groups： control group （n = 10） , model group （n = 20） , nutrition fibre group （n = 20） and salvia miltiorrhiza for enteral nutrition group （n = 20） . The experiment period was 12 days. At the end of experiment, blood routine, biochemistry index （ALB, AST, ALT, BUN, Cr, CK and CK-MB）, the concentrations of IL-6,TNF-α and IL-1β were measured. Results： The model was successful by clinical manifestation, blood routine and pathology. The clinical symptom was improved, blood routine and pathology damage were relieved in salvia miltiorrhiza group. The concentrations of IL-6, TNF-α and IL -1 in salvia miltiorrhiza group were lower than model group and nutrition fibre group （ P 〈 0. 05 ）. Conclusions ： Salvia miltiorrhiza for enteral nutrition could prevent gut function damage, protect intestinal mucosa and decrease the concentrations of pro-inflammatory cytokines.
出处
《肠外与肠内营养》
CAS
北大核心
2009年第4期234-236,238,共4页
Parenteral & Enteral Nutrition
