摘要
目的:观察大承气汤对实验性急性胰腺炎大鼠胰腺腺泡细胞凋亡的影响。方法:36只雄性SD大鼠随机分入12h和24h时间点假手术组、模型组和治疗组,每组6只;其中24只逆行胰胆管注射3.5%牛磺胆酸钠建立急性胰腺炎模型,12只为假手术组。治疗组予大承气汤喷雾干燥颗粒20g/kg灌胃治疗一次,模型组和假手术组给予等量的生理盐水。给药后12h和24h,断头处死取血及胰腺组织,检测胰腺组织中一氧化氮(nitric oxide,NO)含量和诱生型一氧化氮合成酶(inducible NOsynthetase,i NOS)的活性,原位缺口末端标记法检测胰腺组织细胞凋亡率,并观察胰腺组织病理积分。结果:与模型组比较,治疗组两个时间点的血清淀粉酶活性显著降低(P<0.05),胰腺组织内NO含量和i NOS活性明显升高(P<0.05);治疗组大鼠的胰腺腺泡细胞凋亡指数增加,伴随胰腺组织病理学积分的降低(P<0.05)。结论:大承气汤可诱导急性胰腺炎大鼠胰腺腺泡细胞凋亡而改善胰腺病理改变,其机制可能与增加胰腺组织NO含量有关。
Objective： To explore the effects of Dachengqi Decoction （DCQD）. a compound traditional Chmese herbal medicine, on pancreatic acinar cell apoptosis in a rat model of experimental acute pancreatitis. Methods： A total of 36 Sprague-Dawley rats were randomly divided into sham-operated group, untreated group and DCQD-treated group （12 rats in each group）. Acute pancreatitis was induced in 24 rats by retrograde injection of 3.5% sodium taurocholate into pancreatic bile duct. The other 12 rats were allocated as sham-operated group. Alter the operation, the spray-dried DCQD （2 g/mL of crude drugs） or normal saline at 10 mL/kg body weight of rats were orally administered. The rats were sacrificed by decapitation 12 h and 24 h after the administration, and samples were collected. Amylase activity in serum, nitric oxide （NO） content and inducible NO synthetase （iNOS） activity in pancreatic tissue were measured respectively. Pancreatic acinar cell apoptosis was identified by terminal deoxy-nucleot,dyl transterase mediated dUTP nick end-labeling, and pathological scores of pancreatic tissues were determined under a light microscope. Results： At the two time points after treatment, the activities of serum amylase in the treated group were significantly lower than those in the untreated group （P〈0. 05）. while the contents of pancreatic NO and activities of iNOS were higher than those ,n the untreated group （P〈0. 05）. respectively. The pancreaticacinar cell apoptosis rates in the treatment group at 12 h and 24 h were higher than those In the untreeted group, and the mean pancreatic pathomorphologic scores decreased correspondingly （ P〈0.05）. Conclusion： DCQD can induce pancreatic acinar cell apoptosis by increasing NO content and iNOS activity in the pancreas of experimental acute pancreatitis, which helps attenuate the pancreatic pathomorprology.
出处
《中西医结合学报》
CAS
2009年第7期651-656,共6页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金资助项目(No.30400576
No.30672588)
