摘要
目的研究表面活性剂增敏作用下泰乐星（TLS）在碳糊电极（CPE）上的电化学行为，建立一种测定TLS的电化学分析方法。方法用循环伏安法（cyclic voltammetry，CV）和线性扫描伏安法（linear sweep vohammetry，LSV）探讨了TLS在CPE上的电化学行为及其影响因素。结果阴离子表面活性剂十二烷基苯磺酸钠（SDBS）存在下TLS在CPE上有一显著的不可逆氧化峰，峰电位（Ep）为0．735V。结论TLS氧化峰电流与其浓度在1．2×10^-5～1．0×10^-3mol／L范围内呈良好的线性关系，r=0．9974，检出限1．7×10^-7mol／L，以此建立TLS含量的电化学分析方法。测定结果RSD1．6—2．4％，加样回收率98．6％-101．1％。该方法简便快捷，测定结果令人满意。
Objective To research the electrochemical behaviors of tylosin （TLS） based on the enhanced effect of surfactant at carbon paste electrode （CPE）, an electroanalytic method of content determination of TLS was established. Methods Electrochemical behaviors and influence factor of TLS had been investigated by cyclic vohammetry （CV） and linear sweep voltammetry （LSV）. Results Electrochemical results showed that the electrochemical behavior of TLS on surfactant of sodium dodecyl benzene sulfonate in aqueous 0. 10 mol/L PBS exhibited an irreversible electrochemical redox process at CPE, the oxidation peak potential （Ep） was 0.735 V. Conclusion The oxidation peak currents of TLS versus its concentration had a good linear relationship in the concentration range of 1.2×10^-5～ 1.0×10^-3 mol/L with the correlation coefficient of 0. 9974 and the detection limit of 1.7 ×10^-7 mol/L by LSV response. The relative standard deviation was between 1.6% - 2.4 % and recovery was in the range of 98.6% - 101.1%. The method could be applied in the determination of TLS in samples with the satisfied results.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2009年第7期422-424,共3页
Chinese Journal of Antibiotics
