摘要
研究人细胞色素P450(P450,CYP)在大肠杆菌中的功能表达对新药研发,临床药物治疗和药物早期ADME/T性质研究均有重要意义。异源表达人P450使用最多的宿主是大肠杆菌E.coli,然而要获得足量的有催化活性的P450仍是一个难题。结合作者近年研究,对异源表达的研究意义,P450在E.coli中功能表达的策略,高效表达的影响因素和共表达等方面做一评述,指出今后的研究应用方向。
Functional expression of human cytochrome P450 （ P450, CYP） in E. coli is important for new drug R＆D, clinical drug therapy and the study of early ADME/T properties. Escherichia coli is the most extensively utilized host in the production of recombinant human P450 enzymes. However, it is challenging to obtain sufficient P450s with catalytic activity. Depict current developments in the heterologous expression of human P450 enzymes, strategies for the expression of human P450s in E. coli, factors affecting high-level expression, and coexpression, The recent works of authors in expression optimization are also reviewed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第7期94-101,共8页
China Biotechnology
基金
中国博士后科学基金(20080440019)
辽宁省教育厅科研项目(2009A120)资助项目
