摘要
以‘大青叶’(Prunus pseudocerasus var.‘Daqingye’)嫁接甜樱桃‘岱红’(P.avium‘Daihong’)为试材,研究了淹水条件下外源硝态氮对甜樱桃根系蔗糖、葡萄糖、果糖和淀粉含量及蔗糖合酶(SS)合成和分解,蔗糖中性转化酶(NI)和蔗糖酸性转化酶(AI)活性的影响。结果表明,淹水过程中处理与对照根系蔗糖、葡萄糖、果糖和淀粉含量均呈先升后降趋势;与对照相比,外源NO3-提高了根系中蔗糖、葡萄糖、果糖和淀粉的含量,最大值分别比对照提高18.02%、21.97%、25.27%和11.41%。淹水过程中根系SS分解活性呈先升后降趋势,而SS合成活性、NI和AI活性呈下降趋势;加入NO3-后,4种酶活性均高于对照。这些结果表明,淹水条件下外源NO3-可通过调控根系的糖代谢而增强甜樱桃耐低氧能力。
Effects of exogenous NO3 - on sucrose, glucose, fructose and starch contents as well as sucrose synthase （SS） disassemble activity, sucrose synthase synthesis activity, neutral invertase activity （NI） and acid invertase activity （AI） in roots of sweet cherry （Prunus avium ＇ Daihong＇ ） with P. pseudocerasus var. ＇ Daqingye＇ as rootstock under waterlogging condition were studied. The results showed that under water- logging condition, sucrose, glucose, fructose and starch in roots of sweet cherry increased significantly in the early period and then decreased in the later period in both treatment and control. Exogenous NO3 - enhanced the contents of sucrose, glucose, fructose and starch, which were higher than control, while to the maximum, which were 18.02% , 21.97% , 25.27% and 11.41% higher than that of control respectively when reached to the maximum. The sucrose synthase disassemble activity in roots of sweet cherry increased to the highest level and then declined gradually in both treatment and control. Meanwhile, the sucrose synthase synthesis, NI and AI activities decreased gradually, but four enzymes activities of treatment were higher than that of control after using exogenous NO3 - We conclude that the addition of NO3 - can enhance the tolerance of the roots of sweet cherry to hypoxia through regulation of sugar metabolism.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第7期937-944,共8页
Acta Horticulturae Sinica
基金
国家‘十一五’科技支撑重点项目(2008BADA4B05)
山东省良种工程项目
关键词
甜樱桃
根系
硝态氮
淹水
酶活性
sweet cherry
roots
nitrate
waterlogging
enzyme activity
