摘要
白腐真菌F-9是新分离得到的一株白腐真菌,分别采用PDA固体培养基、液体天然培养基和液体人工培养基对它进行培养,并对它的产酶情况进行研究。研究结果表明：F-9在PDA培养基中的生长速率较快,可达到8 mm/d 在液体天然培养基中的生长速率和产酶情况也比较理想,LiP酶活力可达到341 U/L,MnP可达到264 U/L 在液体人工培养基中LiP和MnP活性达到最大的时间比在天然培养基中晚2~3 d,但酶的活力都有很大提高,LiP酶活力可达到619 U/L,MnP酶活力可达到443 U/L。
White-rot fungus F-9 was the fungus isolated newly. Used the PDA culture medium, the natural culture medium on liquid and the artificial culture medium on liquid carries on the raise on F-9 separately, and studied the production of enzyme. The study illustrated that its growth rate in PDA culture medium was quick, may reach 8 mm/d; and its growth rate and production of enzyme in natural culture medium on liquid quit to be also ideal, LiP was 341 U/L, MnP was 264 U/L;the peak activities of LiP and MnP produced by F-9 in the artificial culture medium on liquid appear later than the fungus in natural culture medium on liquid about 2~3 d,but the activities of the two enzyme were both greatly improved,I.iP was 619 U/L,MnP was 443 U/L. Judging from the result, if optimize the train condition of F-9 further, it ought to get much better lignin degradation effect.
出处
《北方园艺》
CAS
北大核心
2009年第7期72-75,共4页
Northern Horticulture
关键词
白腐真菌
生长
产酶
木质素
Whir e-rot fungus F-9
Growth
Enzyme
Lignin
