摘要
通过细胞酶免疫测定法测定了胃上皮细胞与ＨＰ、Ｅ．ｃｏｌｉ、空肠弯曲菌的结合情况，结果表明胃上皮细胞与ＨＰ有较同的结合力，显著高于Ｅ．ｃｏｄｉ、空肠变曲菌组，在工浓度是，反应ＯＤ值随加入菌量的增中而递增，当菌量达１０＾－６－１０＾７／ｍｌ时，ＯＤ值不再递增，提示ＨＰ与胃上皮细胞的结合呈饱和性，特异性，ＨＰ与肝、肠和粘膜细胞结合和低下，提示ＨＰ感染具有组织定向性。
Aim: study on the adhesive character of helicobacter pylori to gastric ep-ithlium cell. Method; the binding capacity of gastric epithlium cell (GEC) to HP, E·coli and campylobacter jejunal were detected by ELISA meanwhile, the adhesive character of HP to liver cell and intesstine mucosa cell were also analyzed. Result: the adhesive character of HP to GEC was significantly enhanced campared with E·coli and campylobacter jejunal to GEC (P<0.05). Moreover, the optical density (OD) is increased with adding bacterium gradually before concentration of bacterial reaching 10~6-10~7/ml. The binding capacity of HP to liver cell and intestine mucosa cell was rather low ( P < 0. 05 ), the result implied that HP conjunct gastric epithilium cell was possessed of saturability and specifici-tv.
出处
《临床消化病杂志》
1998年第2期69-71,共3页
Chinese Journal of Clinical Gastroenterology
关键词
幽门螺杆菌
胃上皮细胞
粘附性
Helicobacter phlori Gastric epithelial cell Adhesive
