摘要
应用多聚酶链反应（ＰＣＲ）检测５１例消化道疾病患者活检或手术标本的人类巨细胞病毒（ＨＣＭＶ）－ＤＮＡ，阳性率９．８０％；采用间接免疫荧光法随机检测其中４１例患者血清ＨＣＭＶ－ＩｇＭ。结果显示：消化道组织巨细胞病毒感染数低于血清ＨＣＭＶ－ＩｇＭ阳性数（Ｐ＜０．０１）；血清ＨＣＭＶ－ＩｇＭ检测中，恶性肿瘤和结肠炎患者ＨＣＭＶ感染均较对照组为高（Ｐ＜０．０１和Ｐ＜０．０５）。
Human cytomegalovirus (HCMV) genomes was detected by PCR in 51 patients' biopsy or operation specimes with gastrointestinal disease. The positive rate was 9.80%. At the same time, 41 patients' serum HCMVIgM was detected by indirect immunofluorescence assay (IFA); the positive rate was 46.34%. Patients with cancer and colitis exhibited a higher HCMV infection rate than those without cancer and colitis. The results suggest that PCR is sensitive and specific in detection of gastrointestinal HCMV infection, and the examination of biopsy or surgical specimen is superior than that of IFA of serum HMCVIgG.
出处
《湖南医科大学学报》
CSCD
1998年第3期229-231,共3页
Bulletin of Hunan Medical University
关键词
巨细胞病毒
消化系疾病
聚合酶链反应
cytomegalovirus
diseases of digestive system
polymerase chain reaction
HIV infection
