摘要
目的探讨在子宫颈上皮内瘤变(CIN)中hTERC(human telomerase RNAgene component)基因荧光原位杂交的拷贝数扩增情况和高危型人乳头状瘤病毒(HR-HPV)检测,作为宫颈癌早期诊断标志物的预测意义。方法应用荧光原位杂交(FISH)方法,检测hTERC基因在72例宫颈脱落细胞中hTERC基因拷贝数的变化,用第二代杂交捕获法(HC-2)检测每一患者的2种HR-HPV DNA(HPV16/18),并同病理诊断结果比较;应用荧光标记探针GLPTERC/CSP3;宫颈癌细胞株Hela和正常骨髓淋巴细胞为阳性对照,正常宫颈细胞为阴性对照。结果在72例脱落细胞中,hTERC基因CINⅡ的阳性表达率为60.00%,CINⅢ的阳性率为83.33%,与正常或炎症/CINⅠ差异有统计学意义(P<0.05);hTERC基因在宫颈癌细胞株HeLa和正常骨髓淋巴细胞中基因扩增阳性;HR-HPV DNA检测阳性32例,炎症/CINⅠ的阳性表达率为42.86%,CINⅡ的阳性表达率为70.00%,CINⅢ的阳性率为66.67%,宫颈癌的阳性率为75.00%;hTERC基因FISH检测对宫颈上皮内瘤变的阳性预测值(PPV)和特异性(Sp)明显高于HC-2HR-HPV DNA检测。结论hTERC基因参与宫颈上皮内瘤变和宫颈癌的发生发展,作为宫颈上皮内瘤变及宫颈癌的标志物之一,TERC基因的FISH检测比HC-2HR-HPV DNA检测能够更可靠地鉴别宫颈良恶性病变,二者联合检测将为提高宫颈癌的早期诊断率提供更有效、更合理的方案。
OBJECTIVE To explore the predictive value of human telomerase RNA gene component （hTERC） gene amplification and high-risk human papilomavirus （HR-HPV） testing in cervical intraepithelial neoplasia （CIN） as a marker for early diagnosis of cervix carcinoma. METHODS Fluorescence in situ hybridization （FISH） was used to detect the amplification of hTERC of cervical epithelial ceils in 72 cases. By using hybrid capture 2（HC-2）, two types of the HR-HPV DNA （HPV16/18） of each case were detected. Then, the results were compared with the pathologic diagnosis. The dual-color probe we used was GLP TERC/CSP 3. HeLa ceils and lymphocytes from normal marrow were the positive control, while the cervical specimens from healthy outpatients were the negative control. RESULTS hTERC Gene amplification of specimens was tested in 72 cases, the positive amplification rate of hTERC gene in the cervicitis/CIN Ⅰ group and normal, compared to the cervical carcinomas （100%） and CINⅡ/Ⅲ （68. 75%）, which showed a significant difference. The rates in CIN Ⅲ and ClN Ⅲwere 60. 00% and 83.33%, respectively, which showed a significant difference compared with normal and CIN Ⅰ/inflammation groups, hTERC gene amplification was positive in both HeLa ceils and lymphocytes from normal marrow and HC- 2 testing was positive in 32 cases of patients containing 11 cases of CIN Ⅱ / Ⅲ , 3 cases of cervical cancer, 18 cases of cervicitis/CIN1 diagnosed. The positive predictive value （PPV） and specificity （Sp） of hTERC for the highgrade CIN was significantly higher than the PPV and Sp of HC-2 HR-HPV testing. CONCLUSIONS hTERC Gene involves in the progression and occurrence of cervical intraepithelial neoplasia and cervical squamous carcinoma. As a marker for early diagnosis of cervical intraepithelial neoplasia and cervical squamous carcinoma, the FISH method for hTERC gene is more reliable to differentiate the malignant diseases from the benign ones in cervixes than HC-2 HR-HPV DNA testing. The combined det
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第14期1838-1841,共4页
Chinese Journal of Nosocomiology
基金
卫生部科研课题(WKJ-2007-3-001)
