摘要
目的调查医院ICU痰标本中分离出鲍氏不动杆菌的耐药特点及产金属β-内酰胺酶情况。方法收集ICU2005年7月-2007年3月经VITEK-32系统GNI+卡鉴定为鲍氏不动杆菌的不重复菌株42株,均为痰标本,用GNS132做常规药物敏感试验,用纸片扩散法(K-B法)补做头孢哌酮/舒巴坦、多黏菌素E、氨曲南的药物敏感试验,用Etest试验检测金属β-内酰胺酶。结果42株鲍氏不动杆菌对18种抗菌药物的耐药率以头孢哌酮/舒巴坦、多黏菌素E最低,为2.4%;亚胺培南的耐药率为66.7%;对其他药物的耐药率为69.4%～100.0%;Etest检出金属β-内酰胺酶阳性菌9株。结论产金属β-内酰胺酶是鲍氏不动杆菌耐亚胺培南的重要机制之一,治疗多药耐药鲍氏不动杆菌引起的感染应首选头孢哌酮/舒巴坦和多黏菌素E。
OBJECTIVE To investigate antimicrobial resistance of Acinetobacter baumannii and detect metallo-β- lactamases （MBLs） in clinical isolates from ICU. METHODS Forty-two strains of A. baumannii were isolated from sputum samples between Jul 2005 and Mar 2007 in Affiliated Hospital of North Sichuan Medical College. Bacteria identification and antimicrobial susceptibility test were performed by VITEK-32 system and K-B disk method. Meanwhile, MBLs were detected by Etest. RESULTS Cefoperazone/sulhactam with low resistance accounted for 2.4%. The resistance to imipenem was 66.7%. The resistance to other antibiotics ranged from 69.4% to 100%. Nine MBLs-produeing strains were detected by Etest. CONCLUSIONS Metallo-β-lactamases produced by A. baumannii are one of important mechanisms which caused resistance to imipenem. Cefoperazone/ sulbactam and polymyxin can be chosen to treat resistant A. baumannii.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第13期1732-1734,共3页
Chinese Journal of Nosocomiology
