摘要
目的研究内皮素、一氧化氮在肝硬变血流动力学紊乱中的作用及关系．方法应用放免法和高效液相色谱法分别检测肝硬变患者４４例，（男３２例，女１２例；年龄５０４岁±１１０岁），其中腹水患者２７例及健康对照２５例（男１８例，女７例；年龄４６８岁±１２４岁）血浆内皮素（ＥＴ）、一氧化氮（ＮＯ）及部分血管活性物质水平．结果肝硬变组血浆ＥＴ及ＮＯ水平（５７０ｎｇ／Ｌ±２５４ｎｇ／Ｌ，３８７２μｇ／Ｌ±１０６４μｇ／Ｌ）明显高于对照组（３３０ｎｇ／Ｌ±１０９ｎｇ／Ｌ，２９２３μｇ／Ｌ±５４５μｇ／Ｌ，Ｐ＜００１）．腹水患者血浆ＥＴ及ＮＯ水平（６７５ｎｇ／Ｌ±２４７ｎｇ／Ｌ，４１４７μｇ／Ｌ±１０７１μｇ／Ｌ），显著高于无腹水患者（４５９ｎｇ／Ｌ±１８３ｎｇ／Ｌ，３２７２μｇ／Ｌ±７０２μｇ／Ｌ，Ｐ＜００１）．ＮＯ与ＥＴ呈直线正相关（ｒ＝０７７２，Ｐ＜００１）．结论肝硬变患者ＥＴ与ＮＯ水平升高，且腹水患者较无腹水者更升高；
AIM To study the effects of endothelin (ET) and nitric oxide (NO) in hemodynamics disturbance of cirrhosis and their relationships. METHODS The plasma levels of ET and NO were measured in 44 patients with cirrhosis (27 cases with ascites) and 25 normal controls by RIA and HPLC. RESULTS The concentrations of ET and NO were significantly higher in patients with cirrhosis ( 57 0ng/L ± 25 4ng/L , 3872μg/L ± 1064μg/L ) than in controls ( 33 0ng/L ± 10 9ng/L , 2923μg/L ± 545μg/L , P <0 01). ET and NO were markedly higher in cirrhosis with ascites ( 67 5ng/L ± 24 7ng/L , 4147μg/L ± 1071μg/L ) than in those without ascites ( 45 9ng/L ± 18 3ng/L , 3272μg/L ± 702μg/L , P <0 01). There was a positive correlation between the levels of NO and ET in cirrhosis ( r =0 772, P <0 01 ). CONCLUSION The plasma levels of ET and NO in cirrhotic patients and in those with ascites were high and positively correlated.
关键词
肝硬化
病理
血液
内皮素
一氧化氮
腹水
liver cirrhosis/pathology
liver cirrhosis/blood
endothelins/blood
nitric oxide/blood
ascites/blood
ascites/pathology
