摘要
研究肉苁蓉主要成分之—─—多糖对小鼠胸腺T细胞的功能调节。方法：采用沉淀法获肉苁蓉粗多糖，以DEAE－SephadexA－50和SephacrylS－200柱层析，获得肉苁蓉多糖（CDPS）。再采用噻唑蓝比色法（MTT）观察CDPS对小鼠脾、胸腺淋巴细胞增殖反应的影响及对正常小鼠脾淋巴细胞分泌IL－2的影响。结果：CDPS纯化后经SephacrylS－200鉴定为单一对称峰。CDPS体外实验，在12．5～200μg／ml浓度范围内，有单独和协同ConA、PHA促进小鼠胸腺淋巴细胞增殖的作用（P＜0．01）。CDPS在100μg／ml浓度时，能显著提高小鼠脾淋巴细胞分泌IL－2的能力．其IL－2活性明显高于对照组（P＜0．01）。结论：肉苁蓉多糖能增加小鼠脾、胸腺淋巴细胞的增殖反应，且与ConA、PHA有协同刺激小鼠胸腺淋巴细胞增殖的作用；并能显著提高小鼠脾脏T淋巴细胞分泌IL－2。
Objective:To investigate immunomodulatory effects on mouse T cells of the polysaccharide Cistanche Deserticola Y C Ma (CDPS). Methods: CDPS were firstly soaked in hot alcohol and purified by chromatography of DEAE-Sephadex A-50 and Sephacryl S-200. The immunomodulatory function of CDPS on mouseT cells was studied in vitro by MTT method. Results :CDPS was identified as homogeneous by Sephacryl S-200.The enhancing effects of CDPS alone and with ConA,PHA on proliferation of lymphocyte from spleen and thymus in vitro were observed (P<0.01 ).Its effect concentration range was 12. 5-200 μg/ml. Higher concentration(100 μg/ml) of CDPS might significantly stimulate secretion of interleukin 2 (IL-2) by lymphocytes. Compared with control group, the reactivity of IL-2 was higher (P<0. 01 ). Conclusion: CDPS can promote the Tlymphocyte proliferation of mice and it has enhancing effect with ConA,PHA;and CDPS may significantly pro-mote secretion of interleukin 2 (IL-2).
基金
国家自然科学基金!39300174
39580025
关键词
肉苁蓉
多糖
小鼠
胸腺
T细胞
Cistanche Deserticola Y C Ma /pharmacol
Polysaccharides / pharmacol
Mice
Thymus gland
T-lymphocytes/drug therapy
