摘要
目的探索水通道蛋白4在脑多形性胶质母细胞瘤中的表达及其与瘤周水肿的关系。方法采用免疫荧光细胞化学及Western blot方法检测30例脑多形性胶质母细胞瘤患者肿瘤组织和瘤周水肿组织标本中AQP4的表达,并分析比较AQP4的表达量与瘤周水肿程度的关系。结果在胶质瘤细胞内,AQP4广泛分布于胞浆内。AQP4在多形性胶质母细胞瘤体及瘤周水肿组织中均表达高于正常对照组(P<0.01),瘤体组和瘤周组之间差异存在统计学意义(P<0.05),瘤周组明显高于瘤体组,且AQP4表达量与瘤周水肿程度呈正相关。结论AQP4在多形性胶质母细胞瘤性脑水肿中表达增高,并与瘤周水肿程度呈正相关,其可能是水肿产生的重要分子基础。多形性胶质母细胞瘤性脑水肿的发生中可能包括细胞毒性和血管源性两种机制。
Objective To investigate the expression of aquaporin - 4 （AQP4） in the brains of patients with glioblastoma and its association with brain edema. Methods Immunofluorescence cytochemistry and western blot tests were performed to detect the expression of AQP4 in the brain tumors and the adjacent tissues in 30 patients with glioblastoma. The association between AQP4 and the extent of brain edema was analysed. Results The AQP4 immunoreactive cells were mainly astrocytes in the brains, which were extensively distributed in the intracytoplasm. Higher expressions of AQP4 were found in the brain tumors and adjacent tissues in the patients with glioblastoma than in the normal controls（P〈0.05）. More AQP4 were distributed in the tumor adjacent tissues than in the tumors （P〈0. 05）. The AQP4 was positively correlated with the extent of brain edema. Conclusion AQP4 overexpress in the brain tumors and adjacent tissues, which is associated with the extent of brain edema. Cytotoxic and vasogenic edemas may coexist in the cerebral edema induced by glioblastoma.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期651-654,共4页
Journal of Sichuan University(Medical Sciences)
