摘要
目的观察黄连素对高脂膳食加小剂量链脲佐菌素（STZ）诱导的2型糖尿病（2-DM）大鼠胰岛素抵抗指数及肾周脂肪组织脂联素基因mRNA表达的影响。方法选用高脂饲料加小剂量STZ建立2-DM大鼠模型共40只，将2-DM大鼠随机分为模型组（水）和黄连素高、中、低剂量组（0．20、0．10、0．05g／kg），每组10只，另选10只大鼠为正常组，各组灌胃8周后，空腹取材，采用逆转录聚合酶链式反应检测肾周白色脂肪组织脂联素基因mRNA水平，放射免疫法测定空腹胰岛素并计算胰岛素抵抗指数（IRI）。结果正常对照组大鼠与2型糖尿病模型组大鼠脂联素基因表达、IRI间比较差异有统计学意义（P〈0．01）；模型组与黄连素高、中剂量组大鼠脂联素基因表达、IRI间差异有统计学意义（P〈0．01）。结论黄连素可增加2-DM大鼠脂联素基因mRNA的表达，降低IRI。
Objective To explore the effect of Berberine decotion on adiponectin gene mRNA expression in white adipose tissue and insulin resistant index of 2-DM induced by hight-fat diat and streptozotocin （STZ）. Methods To build rats model of type 2-DM induced by hight-fat diet and STZ. The rats with 2-DM were divided into four groups randomly：model groups and low, middle, hight doses of berberine groups with 10 in each group,another 10 rats were selected as normol group. After 8 weeks, FINS were detected by radioimmunoassay and was calculated insulin resistant index （ IRI）, mRNA expression of adiponection gene was determinded by RT-PCR. Results There is great statistical difference between control group and 2-DM group about their gene expression of adiponectin and IRI（ P 〈 0.01 ）. So did the model group and berberine group with middle and high dosage. （ P 〈 0.01 ）. Conclusion Berberine can increase mRNA expression of adiponection gene and decrease IRI in 2-DM rats.
出处
《医学综述》
2009年第14期2210-2212,共3页
Medical Recapitulate
关键词
黄连素
2型糖尿病
逆转录-聚合酶链反应
脂联素基因
Bererine
2 diabetic mellitus
Reverse-Transcription polymerase chain reaction
Adiponectin gene
