摘要
目的研究肝细胞生长因子对大鼠供心长时间保存的保护作用并探讨其机制。方法40只SD大鼠采用随机数字表法分为对照组20只和实验组20只，每组再分为2个亚组各10只。建立Langendorff-Neely灌注模型，分别用HTK液和加入重组人肝细胞生长因子100彬L的HTK液作为停搏液和保存液。通过免疫组织化学方法测定c-met受体；末端转移酶标记技术检测细胞凋亡指数；实时聚合酶链反应检测心肌bcl-2mRNA的表达。结果实验组c-met受体表达升高，凋亡指数降低，bcl-2mRNA的表达升高，差异有统计学意义（均P〈0．01）。结论肝细胞生长因子通过其特异性受体c-met的表达上调bcl-2mRNA表达，减轻供心的细胞凋亡，产生有效的保护作用。
Objective To observe the effect of hepatocyte growth factor on isolated rat heart preservation. Methods Forty SD rats were randomly divided into two groups ： control group （ n = 20） with isolated rat heart preserved in 4 ℃ HTK solution and experimental group（n =20） with isolated rat hearts preserved in 4 ℃ HTK ＋ rh-HGF（ 100 μg/L）solution. Cell apoptosis was detected by TUNEL staining and the expression of bcl-2mRNA was examined by RT-PCR. Results Experimental group showed a lower rate of apoptosis cardiomyocytes （ P 〈 0.01 ） after reperfusion. Immunohistochemical analysis revealed that c-met receptor expression was stronger in the HGF- treated myocardium than that in the non-HGF-treated myocardium after storage and was associated with a stronger ex- pression of bcl-2 mRNA. Conclusion The administration of rh-HGF before storage improved cardiac function after prolonged myocardial preservation by preventing apoptosis and enhancing expression of bcl-2 mRNA.
出处
《中国医药》
2009年第7期489-490,共2页
China Medicine
关键词
心脏移植
肝细胞生长因子
心脏保存
Heart transplantation
Hepatocyte growth factor
Heart preservation
