摘要
目的:探讨肝素对肺成纤维细胞增殖的作用。方法:应用细胞计数、3H-TdR掺入和增殖细胞核抗原(PCNA)细胞免疫组化等方法,检测肝素对培养的Wistar乳鼠的肺成纤维细胞(LFb)的作用。结果:肝素可促进LFb增殖且呈量效关系,以0.1mg·L-1作用最强,其次为1mg·L-1,大于10mg·L-1则表现为抑制效应。肝素可协同TNF促LFb增殖。
Purpose To elucidate the effect of heparin on the proliferation of lung fibroblasts (LFb). Methods Cell count, ^(3H)TdR incorporation and PCNA immunostaining were used to investigate the effect of heparin on the proliferation of cultured wistar newborn rat LFb. Results Heparin at low concentration (0.1 mg·L^-1, 1 mg·L^-1) stimulated LFb proliferation where as it inhibit the proliferation when concentration was higher than 10 mg·L^-1. The most proliferative effect was at the concentration of 0.1 mg·L^-1. The effect of TNF on stimulating LFb enhanced by heparin. Conclusion Heparin may play a role on lung fibrogenesis by stimulating proliferation of LFb.
出处
《临床与实验病理学杂志》
CAS
CSCD
1998年第3期276-278,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金