摘要
寄生线虫Ditylenchus destructor是许多作物和蔬菜的重要病原物。本研究进行的密封皿内测定结果表明,由枯草芽孢杆菌BL02和G8产生的挥发物能够强烈地抑制D.destructor(24h杀虫活性均达100%)。经挥发物处理后,大部分线虫在1～12h内活动逐渐减弱,24h后完全停止活动。经固相微萃取并经气相色谱-质谱联用技术进一步鉴定,挥发物包括烷烃、醇、酯、酮、酸、胺、肟、酚及杂环类。本研究为微生物活性挥发物的开发和利用提供了有价值的参考并预示了细菌挥发物在未来植物线虫病害生物防治方面的应用潜力。
Plant-parasitic nematode Ditylenchus destructor is one of the most serious pathogens of crops and vegetables. In this study, volatiles produced by two Bacillus subtilis strains BL02 and G8 both displayed 100% nematicidal activity （NA） against D. destructor in sealed dishes. When exposed to these nematicidal volatiles, the nematodes gradually reduced movement within 1-12 h and stopped completely after 24 h. The effective volatiles were extracted using solid-phase micro-extraction （SPME） and further identified by gas chromatography-mass spectrometry （GC-MS） technique. The detected volatile organic compounds （VOCs） included alkyls, alcohols, esters, ketones, acid, amine, oxime, phenols and heterocyclic compounds. This study provided useful information for the exploitation of microbial bioactive volatiles and was the first report about the potential application of bacterial volatiles in biocontrol of plant-parasitic nematode.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第3期304-309,共6页
Acta Phytopathologica Sinica
基金
National Key Technology R & D Program of China (2006BAD08A03)
Shandong Agricultural University Postdoctor Foundation
