摘要
采用0、1.5、3、6 nmol/L紫杉醇处理A549细胞,建立低浓度紫杉醇诱导非小细胞肺癌凋亡模型,分别采用流式细胞术、细胞计数和免疫印迹法分析细胞凋亡、细胞增殖和Bc1-2家族凋亡相关分子的表达。结果表明,低浓度紫杉醇以浓度和时间依赖的方式诱导细胞凋亡并上调Bim分子表达,而对Bc1-2家族其他凋亡相关分子无显著影响。
A549 cells were treated by O, 1.5,3,6 nmoL/L paclitaxel to establish non-small cell lung cancer apoptosis model,the apoptosis, cell proliferation and expression of apoptosis-related molecules in Bcl-2 family were analyzed with flow cytometry, cell counting and western blot methods. The results showed that paclitaxel with low concn, induced apoptosis in time and concentration-dependent manner, and up-regulated expression of Bim at the same time,but it had no significant effect on other apoptosis-related molecules in Bc1-2 family.
出处
《安徽农业科学》
CAS
北大核心
2009年第20期9323-9325,共3页
Journal of Anhui Agricultural Sciences
