摘要
以健康人外周血单个核细胞作为肿瘤杀伤细胞的前体细胞，ＭＧｃ８０３胃癌细胞作为刺激细胞，在体外进行同种异体淋巴细胞和肿瘤细胞混合培养之后，单独加入重组白介素２和同时加入重组白介素２、抗ＣＤ３单克隆抗体，分别诱导出Ｌ肿瘤杀伤细胞和ＣＤ３肿瘤杀伤细胞．用噻唑蓝比色法在体外检测其对ＭＧｃ８０３胃癌细胞的杀伤活性，并与淋巴因子激活杀伤细胞、ＣＤ３肿瘤杀伤细胞相比较结果显示，ＣＤ３肿瘤杀伤细胞的杀伤活性最高．在倒置显微镜下及透射电镜下观察ＣＤ３肿瘤杀伤细胞对ＭＧｃ８０３胃癌细胞的杀伤过程，发现ＣＤ３肿瘤杀伤细胞首先接触、粘附靶细胞。
Tumor activated killer cells(TAK)were generated in human peripheral blood imononuclear cells by stimulating with human gastric carcinoma cell line MGc803 using allogenetic mixed lymphocyte tumor cell culture method. LTAK cells and CD3TAK cells were induced by MGc803 cells with rIL2, and MGc803 cells with rIL2,and antiCD3Ab,respectively.Testing in vitro cytotoxicity against MGc803 cells in MTT colorimetry CD3TAK cells gave the highest levels of cytotoxity compared with LAK cells and CD3AK cells.In vitro cytolysis process of MGc803 cells mediated by CD3TAK cells were observed using inverted microscopy and transmission electron microscopy.The results showed that CD3TAK cells attached the target and the target cells died by apoptosis and necrosis.
出处
《延边大学医学学报》
CAS
1998年第2期63-65,共3页
Journal of Medical Science Yanbian University
关键词
杀伤细胞
胃肿瘤
免疫疗法
killer cells
stomach neoplasms
immunotherapy
