摘要
目的:探讨细胞因子诱导的杀伤细胞(CIK)抗肿瘤活性,及其作用机制。方法:人宫颈癌细胞HeLa-luc接种BALB/C裸鼠建立动物模型。从健康人外周血单个核细胞诱导培养CIK细胞。CIK或PBS经瘤周注射入荷瘤鼠体内。应用活体生物发光成像技术观察肿瘤大小变化。观察肿瘤的体积和重量变化。ELISA法检测荷瘤鼠外周血中IFN-γ水平。HE染色观察肿瘤、肺脏、肝脏及脾脏的病理形态学变化。结果:CIK治疗后实验组(CIK)肿瘤明显比对照组(PBS)体积小,P<0.05。接种HeLa-luc细胞第5、8周的抑瘤率分别为47.18%、64.38%。接种HeLa-luc细胞第8周实验组血清中IFN-γ〔(61.92±6.49)ρg/mL〕明显高于对照组〔(34.30±1.78)ρg/mL〕,P<0.01。实验组和对照组中,肿瘤组织形态学无明显差别。实验组肺脏、肝脏未见癌结节出现,但均有炎细胞浸润。对照组肺脏出现大量癌结节而肝脏未见癌结节出现。两组脾脏中均可见癌结节。结论:CIK细胞可以明显抑制肿瘤的生长,其机制可能与免疫细胞产生IFN-γ有关。该研究为肿瘤的临床过继免疫治疗提供了理论依据。
OBJECTIVE：To discuss the anti tumor activity of cy tokine-induce killer（CIK） cells re-infused to mice inoculated with cervi cal cancer cells and its related mechanisms. METHODS： The BALB/C nude mice inoculated with cervical cancer cell line HeLa-luc were used as an animal model. The CIK cells from peripheral blood mononuclear cells of healthy donor were cultured. The CIK cells or PBS were re-in- fused through peri-tumor. Tumor changing was detected by using in vi- vo biouminescence imaging. The tumor size and weight were recorded for evaluation. The level of IFN-γ in peripheral blood of bearing tumor mice was detected by ELISA. The histopathological changes of the tumor, lung, liver and spleen were observed by HE staining. RESULTS： The tumor size in the experiment group was smaller than that in the control group after CIK treatment （P〈0.05）. The inhibitory rates of the fifth week and eighth week were 47.18~ and 64.38~ re spectively after being inoculated with cervical cancer cell line HeLa-luc. The level of IFN 7 in the experiment group [（61.92±6.49） pg/mL] was higher than that in the control group [（34.30±1.78） pg/mL] on the eighth week after being inoculated with cervical cancer cell line He- La luc （P〈0.01）. The histopathological changes of tumor had no sig nificantly difference in the two groups. In the experiment group, cancer nodules were not found in the lung and liver, but there were many in- flammatory cells. Many cancer nodules were found in the lung and it was not found in the liver in the control group. Cancer nodules were found in the spleen in the two groups. CONCLUSIONS： The CIK cells show potent anti tumor activity in animal experiment, and its mecha- nisms is related to IFN -γ. This study can provide a theroretical basis in clinical adoptive immunotherapy.
出处
《中华肿瘤防治杂志》
CAS
2009年第11期825-828,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
河北省卫生厅科研基金项目(07265)
河北省普通高等学校强势特色学科基金资助项目(20052)
